CUGBP Elav-like family member 2

Protein information

  • Protein ID: O95319
  • Protein Name : CUGBP Elav-like family member 2
  • Gene : CELF2
  • Organism : Homo sapiens
  • Status : reviewed
  • Function : RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of TNNT2 in embryonic but not adult skeletal muscle. Activates TNNT2 exon 5 inclusion by antagonizing the repressive effect of PTB. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre-mRNAs. Promotes inclusion of exonS 21 and exclusion of exon 5 of the NMDA receptor R1 pre-mRNA. Involved in the apoB RNA editing activity. Increases COX2 mRNA stability and inhibits COX2 mRNA translation in epithelial cells after radiation injury (By similarity). Modulates the cellular apoptosis program by regulating COX2-mediated prostaglandin E2 (PGE2) expression (By similarity). Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK. Binds to the muscle-specific splicing enhancer (MSE) intronic sites flanking the TNNT2 alternative exon 5. Binds preferentially to UG-rich sequences in particular UG repeat and UGUU motifs. Binds to apoB mRNA specifically to AU-rich sequences located immediatly upstream of the edited cytidine. Binds AU-rich sequences in the 3'-UTR of COX2 mRNA (By similarity). Binds to an intronic RNA element responsible for the silencing of exon 21 splicing (By similarity). Binds to (CUG)n repeats (By similarity). May be a specific regulator of miRNA biogenesis. Binds to primary microRNA pri-MIR140 and with CELF1 negatively regulates the processing to mature miRNA (PubMed:28431233).

Protein Sequence

  • MRCPKSAVTMRNEELLLSNGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELFEPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKPADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFSTRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY

RRM domains in this Protein - 3 

RRM Entryname RRM Sequence range Pfam ID
O95319_RRM1 42 - 113 PF00076
O95319_RRM2 134 - 202 PF00076
O95319_RRM3 425 - 495 PF00076

RRM domain Linkers in this Protein - 2 

Linker Name Linker Sequence range
O95319_linker1 114 - 133
O95319_linker2 203 - 424

Experiments retrieved for this Protein - 6 

Experiment ID Experiment Type PubMed ID
Exp_2MY7 NMR 31866002
Exp_2MY8 NMR 31866002
Exp_4LJM X-ray Diffraction
Exp_4LMZ X-ray Diffraction
Exp_4TLQ X-ray Diffraction
Exp_5M8I NMR 28935965

Ligands tested for Binding - 2 

Ligand ID Ligand Type Ligand Sequence
Lig115 RNA GCUGCGUAUUGUUUG
Lig190 RNA UUUAA