Protein NAM8

Protein information

  • Protein ID: Q00539
  • Protein Name : Protein NAM8
  • Gene : NAM8
  • Organism : Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
  • Status : reviewed
  • Function : Component of the U1 small nuclear ribonucleoprotein complex (U1 snRNP) involved in the initiation of meiotic recombination (PubMed:1603056 PubMed:7625279). Involved in the formation of DSBs at recombination hot-spots through meiosis-specific splicing of REC107 pre-mRNA (PubMed:7625279 PubMed:9112441 PubMed:21788335). Collaborates with MER1 to promote splicing of essential meiotic mRNAs REC10 AMA1 MER3 HFM1 SPO22 and PCH2 (PubMed:10983980 PubMed:21208980 PubMed:21788335). NAM8 interacts with the pre-mRNA downstream of the 5' splice site in a region of non-conserved sequence and is required for efficient splicing of uncapped RNA precursor (PubMed:10072385).

Protein Sequence

  • MSYKQTTYYPSRGNLVRNDSSPYTNTISSETNNSSTSVLSLQGASNVSLGTTGNQLYMGDLDPTWDKNTVRQIWASLGEANINVRMMWNNTLNNGSRSSMGPKNNQGYCFVDFPSSTHAANALLKNGMLIPNFPNKKLKLNWATSSYSNSNNSLNNVKSGNNCSIFVGDLAPNVTESQLFELFINRYASTSHAKIVHDQVTGMSKGYGFVKFTNSDEQQLALSEMQGVFLNGRAIKVGPTSGQQQHVSGNNDYNRSSSSLNNENVDSRFLSKGQSFLSNGNNNMGFKRNHMSQFIYPVQQQPSLNHFTDPNNTTVFIGGLSSLVTEDELRAYFQPFGTIVYVKIPVGKCCGFVQYVDRLSAEAAIAGMQGFPIANSRVRLSWGRSAKQTALLQQAMLSNSLQVQQQQPGLQQPNYGYIPSSTCEAPVLPDNNVSSTMLPGCQILNYSNPYANANGLGSNNFSFYSNNNATNTQATSLLADTSSMDLSGTGGQQVIMQGSEAVVNSTNAMLNRLEQGSNGFMFA

RRM domains in this Protein - 2 

RRM Entryname RRM Sequence range Pfam ID
Q00539_RRM1 165 - 236 PF00076
Q00539_RRM2 315 - 379 PF00076

RRM domain Linkers in this Protein - 1 

Linker Name Linker Sequence range
Q00539_linker1 237 - 314

Experiments retrieved for this Protein - 7 

Experiment ID Experiment Type PubMed ID
Exp_5ZWN EM 29794219
Exp_6G90 EM 29995849
Exp_6N7P EM 31485080
Exp_6N7R EM 31485080
Exp_6N7X EM 29051543
Exp_7OQC EM 34349264
Exp_7OQE EM 34349264

Ligands tested for Binding - 2 

Ligand ID Ligand Type Ligand Sequence
Lig180 RNA AUACUUACCUUAAGAUAUCAGAGGAGAUCAAGAAGUCCUACUGAUCAAACAUGCGCUUCCAAUAGUAGAAGGACGUUAAGCAUUUAUCAUUGAACUAUAAUUGUUCAUUGAAGUCAUUGAUGCAAACUCCUUGGUCACACACACAUACGGCGCGGAAGGCGUGUUUGCUGACGUUUCCAUUCCCUUGUUUCAAUCAUUGGUUAAUCCCUUGAUUCCUUUGGGGAUUUUUGGGUUAAACUGAUUUUUGGGGCCCUUUGUUUCUUCUGCCUGGAGAAGUUUGACACCAAAUUCAAAUUGGUGUUAGGGGAGCUGGGGCCUUUCAAAAGAGAGCUUUGUAGAGGCAUUCUUUUUGACUACUUUUCUCUAGCGUGCCAUUUUAGUUUUUGACGGCAGAUUCGAAUGAACUUAAGUUUAUGAUGAAGGUAUGGCUGUUGAGAUUAUUUGGUCGGGAUUGUAGUUUGAAGAUGUGCUCUUUUGAGCAGUCUCAACUUUGCUCGUUCCCGUUAUGGGAAAAAUUUUGGAAGGUCUUGGUAGGAACGGGUGGAUCUUAUAAUUUUUGAUUUAUUUU
Lig23 RNA GGGAACUAAGUGAUCUAGAAAGGUAUGUCUAAAGUUAUGGCCACGUUUCAAAUGCGUGCUUUUUUUUUAAAACUUAUGCUCUUAUUUACUAACAAAAUCAACAUGCUAUUGAACUAGAGAUCCACCUACUUCAUGUUCAGCCGGUCCAGGAUCCGAUAUCCGUACACCAUCAGGGUACGAGCUAGCCCAUGGCGUACACCAUCAGGGUACGACUAGUAGAUCUCGUACACCAUCAGGGUACGGAAUUCUCUAG