P26599_RRM1

Protein information

  • Protein ID: P26599
  • Protein Name: Polypyrimidine tract-binding protein 1
  • Organism: Homo sapiens
  • Gene Name: PTBP1

Domain information

  • Pfam ID: PF13893
  • Pfam Clan Name: RRM
  • Domain Range: 38 - 152
  • Domain Length: 115

Domain Sequence

  • AANGNDSKKFKGDSRSAGVPSRVIHIRKLPIDVTEGEVISLGLPFGKVTNLLMLKGKNQAFIEMNTEEAANTMVNYYTSVTPVLRGQPIYIQFSNHKELKTDSSPNQARAQAALQ

Available Structures for this domain - 3 

Experiment ID RRM Structure Experiment Type Ligand ID Contacts
Exp_1SJQ 1SJQA01 NMR No contacts
Exp_2AD9 2AD9A01 NMR Lig223 Contacts
Exp_2N3O 2N3OA01 NMR Lig11 Contacts

Binding Experiments for this domain - 3 

Experiment ID RRM Entryname Experiment Type Ligand ID Binds? Binding Affinity
Exp_253 P26599_RRM1 Filter binding assay Lig328 Yes 0.026 ± μM
Exp_259 P26599_RRM1 Filter binding assay Lig232 Yes 0.038 ± μM
Exp_262 P26599_RRM1 Filter binding assay Lig301 Yes 0.061 ± μM

Ligands tested for Binding - 5 

Ligand ID Ligand Type Ligand Sequence
Lig11 RNA GGGACCUGGUCUUUCCAGGUCCC
Lig223 RNA CUCUCU
Lig232 RNA UUGAAAGCCGGGGGUGGGAGAUCCGGAUUGCCAGUCUGCUCGAUAUCGCAGGCUGGGUCCGUGACUACCCACUCCCCCUUUCAACGUGAAGGCUACGAUAGUGCCAGGGCGGGUACUGCCGUAAGUGCCACCCCAAAAUAACAACAGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCUCUCCCUCCCCCCCCCCUAACGUUACUGGCCGAAGCCGCUUGGAAUAAGGCCGGUGUGCGUUUGUCUAUAUGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGAAACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGCAAGGUCUGUUGAAUGUCGUGAAGGAAGCAGUUCCUCUGGAAGCUUCUUGAAGACAAACAACGUCUGUAGCGACCCUUUGCAGGCAGCGGAACCCCCCACCUGGCGACAGGUGCCUCUGCGGCCAAAAGCCACGUGUAUAAGAUACACCUGCAAAGGCGGCACAACCCCAGUGCCACGUUGUGAGUUGGAUAGUUGUGGAAAGAGUCAAAUGGCUCUCCUCAAGCGUAUUCAACAAGGGGCUGAAGGAUGCCCAGAAGGUACCCCAUUGUAUGGGAUCUGAUCUGGGGCCUCGGUGCACAUGCUUUACAUGUGUUUAGUCGAGGUUAAAAAACGUCUAGGCCCCCCGAACCACGGGGACGUGGUUUUCCUUUGAAAAACACGAUGAUAAUAUGGCCACAACCAUGGAACAAGAGACUUGCGCGCACUCUCUCACUUUUGAGGAAUGCCCAAAAUGCUCUGCUCUACAAUACCGUAAUGGAUUUUACCUGCUAAAGUAUGAUGAAGAAUGGUACCCAGAGGAGUUAUUGACUGAUGGAGAGGAUGAUGUCUUUGAUCCCGAAUUAGACAUGGAAGUCGUUUUCGAGUUACAGGGCAAUUCCACCUCCUCAGACAAGAAUAACUCCUCCUCGGAAGGCAAUGAAGGUGUGAUCAUCAAUAACUUUUACUCCAACCAAUAUCAAAACUCCAUUGACCUCUCUGCUAAUGCAGCCGGGUCUGACCCACCCAGAACCUACGGUCAAUUUUCGAAUCUCUUUUCGGGCGCAGUGAAUGCCUUUUCUAAUAUGCUUCCAUUGCUAGCUGAUCAAAAUACAGAAGAAAUGGAGAAUCUGUCUGAUCGAGUGUCUCAAGACACUGCCGGCAAUACGGUCACAAACACCCAGUCAACAGUGGGCCGUCUUGUCGGUUAUGGUACCGUUCAUGAUGGAGAGCAUCCGGCAUCAUGUGCUGACACUGCUUCAGAAAAGAUCCUGGCGGUGGAAAGGUACUACACCUUCAAGGUUAAUGAUUGGACAUCAACACAAAAGCCCUUUGAGUACAUCCGCAUUCCCCUUCCUCACGUCCUGUCCGGUGAAGAUGGUGGUGUCUUUGGUGCGGCCCUCCGCCGGCACUACCUGGUGAAAACUGGAUGGCGGGUGCAAGUUCAGUGCAACGCCUCUCAAUUCCACGCUGGAGGUUUGCUGGUGUUCAUGGCACCAGAGUAUCCAACCUUAGAUGCUUUUGCCAUGGACAACCGUUGGUCCAAGGAUAACCUGCCUAAUGGAACCAGAACUCAGACAAACAAAAAGGGACCAUUUGCCAUGGACCAUCAGAACUUCUGGCAGUGGACCUUGUAUCCCCAUCAAUUCCUGAAUCUGAGAACUAACACCACAGUGGAUCUUGAGGUGCCAUAUGUAAACAUAGCCCCCACUUCCUCCUGGACACAACAUGCUUCCUGGACUUUGGUGAUUGCAGUGGUUGCUCCCCUGACAUACUCAACCGGGGCUUCUACCAGUUUGGAUAUCACCGCUUCUAUUCAGCCAGUAAGGCCUGUCUUUAAUGGCCUCCGGCAUGAGACACUUUCUAGACAGUCGCCCAUUCCGGUCACAAUUAGAGAACAUGCUGGUACCUGGUAUUCUACUCUGCCAGACAGCACAGUGCCUAUUUAUGGCAAGACUCCUGUUGCUCCAUCCAAUUACAUGGUAGGCGAAUACAAGGACUUCCUGGAGAUAGCUCAGAUUCCAACCUUUAUUGGGAAUAAGAUCCCUAAUGCUGUCCCCUACAUUGAGGCAUCCAACACAGCCGUCAAGACCCAACCGCUGGCCACCUAUCAAGUGACCUUGUCCUGCUCCUGUCUGGCCAAUACAUUCUUGGCCGCUUUGUCUAGAAACUUUGCUCAGUACCGGGGAUCAUUGGUUUAUACCUUUGUGUUCACUGGGACCGCGAUGAUGAAGGGCAAGUUCCUCAUUGCCUACACCCCACCUGGAGCGGGCAAGCCCACUAGUCGAGACCAAGCCAUGCAGGCGACUUAUGCGAUUUGGGAUUUGGGGCUAAAUUCUUCUUACUCCUUCACUGUGCCUUUUAUUUCUCCCACUCACUUCCGCAUGGUAGGUACUGACCAAGUCAACAUCACUAAUGCGGAUGGCUGGGUUACCGUGUGGCAGCUCACUCCCCUCACUUACCCACCAGGAUGCCCGACCUCUGCUAAGAUACUAACAAUGGUGAGCGCAGGGAAGGAUUUCUCACUCAAGAUGCCUAUCUCACCUGCCCCCUGGAGCCCUCAGGGAGUAGAAAACGCUGAAAAAGGGGUCACUGAAAACACAAACGCAACUGCUGACUUUGUGGCUCAACCAGUUUACUUGCCUGAGAACCAAACGAAGGUGGCUUUCUUCUAUAAUAGGUCCAGUCCCAUUGGUGCCUUCACCGUGAAGUCCGGCAGUCUAGAAUCUGGUUUUGCCCCGUUCUCUAAUGGGACUUGCCCGAACUCAGUGAUACUGACCCCUGGGCCCCAAUUUGACCCCGCCUAUGACCAACUCAGGCCACAGCGUCUGACAGAAAUUUGGGGCAAUGGAAAUGAGGAGACCUCAAAAGUCUUUCCGCUUAAAUCCAAACAGGAUUAUUCCUUCUGCCUCUUCUCCCCCUUUGUGUAUUAUAAAUGUGAUUUAGAAGUGACUCUUAGUCCUCACACUUCAGGCAACCAUGGGCUGUUGGUGAGGUGGUGUCCCACUGGUACACCAACCAAGCCCACUACCCAGGUUCUCCAUGAAGUAAGUUCCCUCUCAGAAGGCAGAACCCCCCAGGUUUAUAGUGCCGGACCUGGCAUUUCAAAUCAGAUUUCCUUUGUAGUUCCUUACAAUUCUCCACUUUCAGUCCUAUCAGCUGUCUGGUAUAAUGGACACAAGAGAUUUGACAACACUGGGAGCUUGGGCAUUGCCCCUAAUUCUGAUUUCGGCACUCUGUUCUUUGCUGGCACAAAGCCUGACAUUAAAUUCACAGUCUACUUGAGAUACAAGAAUAAGAGAGUUUUUUGCCCACGUCCGACUGUCUUUUUCCCCUGGCCCACUUCCGGAGACAAGAUUGAUAUGACCCCGAGAGCUGGAGUCUUGAUGCUAGAGAGUCCAAAUGCCCUAGACAUUUCAAGAACAUACCCCACGUUACAUGUUCUCAUUCAAUUCAACCAUAGAGGUUUGGAGGUUAGAUUGUUUAGACAUGGACACUUUUGGGCUGAAACACGUGCGGACGUGAUUCUGAGAUCAAAGACCAAACAGGUCUCUUUCCUGAGCAACGGGAACUACCCGUCAAUGGACUCUAGAGCUCCCUGGAAUCCUUGGAAGAAUACCUACCAGGCGGUUCUAAGAGCAGAACCAUGUAGAGUGACCAUGGAUAUAUAUUAUAAGAGAGUCAGGCCUUUUAGACUGCCCCUGGUUCAGAAGGAAUGGCCCGUGCGAGAGGAGAACGUUUUCGGUUUGUACCGGAUCUUCAAUGCCCACUACGCUGGUUACUUUGCGGACCUACUGAUUCAUGACAUUGAGACAAAUCCAGGGCCCUUCAUGUUUAGACCAAGGAAACAGGUUUUCCAGACCCAAGGAGCGGCAGUGUCAUCAAUGGCUCAAACCCUACUGCCGAACGACCUUGCCAGCAAAGCUAUGGGAUCAGCUUUUACGGCUUUGCUCGAUGCCAACGAGGACGCCCAAAAAGCAAUGAAGAUUAUAAAGACAUUAAGUUCUCUAUCGGAUGCAUGGGAAAAUGUAAAAGAAACACUAAACAACCCAGAGUUCUGGAAGCAGCUCUUGAGCAGAUGUGUGCAGCUGAUUGCAGGGAUGACAAUAGCAGUGAUGCAUCCGGACCCUUUGACUCUGCUCUGCUUAGGAACAUUGACGGCCGCCGAGAUUACAAGCCAGACAAGUCUGUGCGAAGAAAUAGCAGCUAAGUUCAAGACAAUUUUCAUCACUCCUCCACCACGGUUUCCCACAAUCUCUCUUUUCCAACAACAAUCCCCCUUGAAACAGGUAAAUGAUAUUUUCUCCCUAGCCAAGAACCUGGACUGGGCCGUCAAGACUGUGGAAAAGGUGGUUGAUUGGUUUGGGACAUGGAUAGUACAGGAGGAAAAGGAACAGACCCUAGAUCAGCUCUUGCAGCGUUUCCCCGAACAUGCGAAGCGCAUUUCUGAUCUCCGGAAUGGAAUGGCCGCCUAUGUAGAGUGCAAGGAGAGUUUUGAUUUCUUUGAAAAGCUGUACAAUCAGGCAGUGAAAGAGAAGAGAACGGGUAUCGCCGCCGUCUGUGAAAAAUUCAGACAGAAGCAUGACCACGCCACCGCUCGGUGUGAGCCAGUCGUGAUUGUGCUCCGCGGAGACGCGGGGCAAGGGAAAUCUUUAUCAAGUCAGGUUAUUGCCCAGGCCGUCUCCAAGACCAUUUUCGGCCGGCAAUCUGUGUAUUCCCUUCCCCCCGAUUCGGAUUUCUUUGAUGGCUAUGAAAAUCAGUUUGCAGCAAUAAUGGAUGAUCUAGGGCAAAAUCCUGAUGGCUCUGAUUUCACUACGUUCUGUCAGAUGGUUUCGACUACCAAUUUUCUCCCCAAUAUGGCUAGUCUAGAGAGAAAGGGCACCCCCUUUACAUCUCAGCUUGUGGUGGCAACUACCAAUCUGCCUGAGUUUAGACCUGUCACAAUAGCCCAUUACCCUGCUGUUGAGAGAAGGAUAACUUUCGACUAUUCAGUGUCUGCUGGUCCAGUCUGCUCCAAAACAGAGGCCGGGUAUAAGGUUUUGGAUGUUGAAAGAGCCUUUAGGCCUACCGGUGAGGCUCCUCUUCCAUGCUUCCAGAAUAACUGCCUUUUCCUUGAGAAAGCUGGGCUCCAGUUCAGAGAUAACCGAACUAAAGAGAUCAUUUCCCUGGUAGAUGUGAUUGAGAGAGCCGUGGCUAGGAUUGAAAGGAAGAAGAAAGUUCUCACAACCGUGCAGACCCUUGUGGCACAAGGUCCAGUAGACGAGGUCAGUUUCCAUUCCGUAGUCCAGCAGCUUAAAGCAAGACAGCAAGCGACAGAUGAACAGCUUGAGGAAUUGCAAGAGGCUUUCGCGAAAGUACAGGAGCGUAACUCUGUGUUUUCUGAUUGGUUGAAGAUUUCUGCAAUGUUGUGUGCUGCGACUCUGGCACUUUCCCAAGUUGUCAAGAUGGCCAAGGCGGUGAAGCAGAUGGUCAAGCCUGAUCUGGUUCGUGUGCAAUUGGAUGAGCAGGAGCAGGGACCUUACAAUGAGACAGCGAGAGUUAAACCAAAAACACUGCAGUUGUUGGACAUUCAGGGACCAAACCCUGUGAUGGACUUUGAAAAAUAUGUAGCCAAACAUGUAACCGCCCCCAUUGGUUUUGUCUACCCCACUGGGGUGAGCACCCAGACUUGCCUCCUUGUGAGAGGCCGCACCUUGGUAGUAAAUAGACACAUGGCCGAGUCUGACUGGACUUCCAUAGUAGUGCGUGGAGUCACACACGCCCGCUCUACUGUUAAAAUUUUGGCCAUAGCUAAAGCAGGCAAAGAGACUGACGUAUCUUUCAUCCGCCUCUCUUCUGGUCCUCUAUUCAGAGACAAUACAUCCAAAUUUGUCAAGGCUGGUGAUGUACUUCCUACUGGUGCCGCUCCAGUCACGGGGAUAAUGAACACGGACAUACCCAUGAUGUACACAGGAACCUUCCUGAAAGCUGGUGUGUCAGUCCCAGUGGAAACCGGCCAGACCUUUAAUCACUGUAUUCAUUACAAGGCUAACACACGAAAGGGCUGGUGUGGAUCAGCCCUACUGGCAGAUCUUGGAGGAAGCAAGAAAAUCCUUGGCAUCCAUUCUGCUGGCUCUAUGGGAAUAGCCGCCGCCUCGAUUGUGUCACAGGAGAUGAUUCGGGCGGUAGUGAAUGCCUUUGAGCCACAGGGUGCUCUCGAGAGAUUGCCAGAUGGGCCCCGUAUUCACGUACCACGUAAAACAGCACUACGCCCCACCGUUGCCCGUCAAGUCUUCCAACCAGCAUAUGCCCCGGCUGUUCUAUCGAAAUUUGACCCUAGAACAGAGGCUGAUGUAGAUGAAGUGGCUUUCUCCAAACAUACCUCCAACCAGGAAAGCCUCCCACCAGUGUUUAGAAUGGUAGCCAAAGAGUAUGCCAAUAGAGUUUUCACCUUGCUGGGAAAAGACAAUGGCCGUCUGACUGUAAAGCAGGCUUUGGAAGGACUGGAGGGGAUGGACCCCAUGGACAGGAACACCUCCCCGGGGCUUCCAUAUACUGCGCUAGGAAUGCGCAGAACAGAUGUCGUAGAUUGGGAAUCAGCCACCCUGAUCCCGUUUGCGGCAGAAAGAUUAAGAAAAAUGAAUGAAGGAGACUUUUCCGAAGUUGUCUAUCAAACAUUCCUCAAGGAUGAGCUUAGACCGAUAGAGAAGGUUCAAGCCGCCAAGACACGGAUUGUAGAUGUUCCACCAUUUGAGCAUUGCAUUCUGGGUAGACAAUUGUUGGGAAAGUUUGCAUCAAAGUUCCAGACCCAACCGGGUCUGGAACUAGGAUCAGCCAUUGGAUGUGACCCAGAUGUACACUGGACUGCCUUCGGUGUCGCCAUGCAAGGUUUUGAGCGUGUCUACGAUGUGGACUACUCCAACUUUGAUUCGACCCAUUCGGUGGCAAUGUUCCGCUUAUUGGCUGAGGAAUUUUUCACUCCAGAGAAUGGUUUUGACCCCCUGACUAGAGAAUAUCUUGAGUCAUUAGCCAUUUCAACCCAUGCGUUUGAGGAGAAGCGCUUUCUGAUAACCGGUGGUCUCCCAUCAGGUUGUGCAGCGACCUCAAUGCUAAACACUAUAAUGAAUAAUAUAAUAAUUAGGGCGGGUUUGUAUCUCACGUAUAAAAAUUUUGAAUUUGAUGAUGUGAAGGUGUUGUCGUACGGAGAUGAUCUCCUUGUGGCCACAAAUUACCAAUUGGAUUUUGAUAAGGUGAGAGCAAGCCUCGCAAAGACAGGAUAUAAGAUAACUCCCGCUAACACAACUUCUACCUUUCCUCUUAAUUCGACGCUUGAAGACGUUGUCUUCUUAAAAAGAAAGUUUAAGAAAGAGGGCCCUCUGUAUCGGCCUGUCAUGAACAGAGAGGCGUUGGAAGCAAUGUUGUCAUACUAUCGUCCAGGGACUCUAUCUGAGAAACUCACUUCGAUCACUAUGCUUGCCGUUCAUUCUGGCAAGCAGGAAUAUGAUCGGCUCUUUGCCCCAUUCCGUGAGGUAGGGGUUGUCGUGCCAUCAUUCGAGAGUGUGGAGUACAGAUGGAGGAGUCUGUUCUGGUAGUAGUGUAGUCACUGGCACAACGCGUUACCCGGUAAGCCAAUCGGGUAUACACGGUCGUCAUACUGCAGACAGGGUUCUUCUACUUUGCAAGAUAGUCUAGAGUAGUAAAAUAAAUAGAUAGAG
Lig301 RNA UUGAAAGGGGGCGCUAGGGUUUCACCCCUAGCACGCCAACGACAGUCCCUGCGUUGCACUCCACACUUACGUUCGCGUGCACGCGGGACCCGAUGGACUUUCGUUCACCCACCUACGGUUGGACUCACGGCACCGCGUGGCCAUUUUAGCUGGACCGUGUGGACGAACACCGCUUGCGCUUCUCGCGUGACCGGCUAGUACUCUGACCACUCUCCGCCUACUUGGUCGCUGCGCUGUCUUGGGCACUCCUAUUGGGGUCGUUCGACGCUCCACGGGCUCCCCCGUGCGGCGGGCUACGGUGAUGGGGCCGUUUCGCGCGGGCUGAUCGCCUGGUCUGCUUCGGCUGUCACCCGAAGCCCGCCUUUCACCCCCCCCCCCCUAAGUUUUACCGUCGUUUCCGACGUAAAGGGGAAGUAACCACAAGCUUGACACCGCCUGUCCCGGCGUUAAAGGGAAGUAACCACAAGCUUACAACCGCCUACCCCGGUGUUAAUGGGAUGUAACCACAAGAUACACCUUCACCCGGAAGUAAAACGGCAAAUUCACACAGUUUUGCCCGUUUUUCAUGAGAAACGGGACGUCUGCGCACGAAACGCGCCGUCGCUUGAGGAGGACUUGUACAAACACGAUCUAAACAGGUUUCCCCAACUGACAUACACCGUGCAAUUUGAAACUCCGCCUGGUCUUUCCAGGUCUAGAGGGGUAACACUUUGUACUGUGCUUGACUCCACGCUCGGUCCACUGGCGAGUGUUAGUAACAGCACUGGUGCUUCGUAGCGGAGCAUGGUGGCCGUGGGAACUCCUCCUUGGUAACAAGGACCCACGGGGCCGAAAGCCACGUCCUGACGGACCCACCAUGUGUGCAACCCCAGCACGGCAACUUUUCUGUGAAACUCACUCUAAGGUGACACUGAUACUGGUAUUCAAGUACUGGUGACAGGCUAAGGAUGCCCUUCAGGUACCCCGAGGUAACACGCGACACUCGGGAUCUGAGAAGGGGACUGGGGCUUCUGUAAAAGCGCCCAGUUUAAAAAGCUUCUAUGCCUGGAUAGGUGACCGGAGGCCGGCGCCUUUCCAUUAUAACUACUGACUUUAUGAACACAACUAACUGUUUUAUCGCUUUGGUGUACGCUAUCAGAGAGAUCAGAACGCUUUUCCUUUCACGAGCUACAGGAAAGAUGGAAUUCACGCUGUACAACGGUGAAAAGAAGACCUUUUACUCCAGGCCCAACAACCACGACAACUGUUGGUUGAACACCAUCCUUCAGUUGUUCAGGUACGUCGAUGAGCCUUUCUUCGACUGGGUCUACAACUCACCCCAGAACCUCACGCUUGAAGCCAUCAAACAGCUGGAAGAACUCACAGGGCUUGAGUUACACGAAGGCGGACCGCCUGCCGCUGUUGUUUGGAACAUCAAACACUUGCUUCAAACCGGUAUUGGUACCGCCUCGCGACCUAGCGAGGUGUGUGUGGUGGACGGUACGAACAUGUGUCUGGCUGAUUUCCAUGCAGGCAUCUUCCUGAAAGGACAGGAACAUGCCGUGUUUGCUUGUGUCACCUCCAACGGGUGGUACGCGAUUGACGAUGAGGACUUCUACCCAUGGACGCCGGACCCGUCCGACGUUCUGGUGUUCGUUCCAUAUGAUCAAGAACCACUCAACGGAGAAUGGAAAAUCAACGUUCAGCGGAAGCUCAAGGGAGCUGGACAAUCCAGUCCGGCGACCGGCUCGCAGAACCAAUCUGGCAAUACUGGCAGCAUAAUCAACAACUACUACAUGCAACAGUACCAGAACUCCAUGGACACACAACUUGGUGACAACGCCAUCAGCGGAGGCUCCAACGAGGGCUCCACAGACACAACUUCCAAUCACACAACCAACACUCAGAACAACGACUGGUUUUCCAAGCUCGCAGGUUCUGCCUUCAGCGGUCUUUUUGGCGCCCUUCUUGCUGACAAAAAGACAGAGGAAACCACUCUCCUCGAGGACCGCAUCCUCACUACCCGCAACGGACACACGACCUCAACAACACAGUCGAGUGUCGGAGUCACGUACGGGUAUGCAACAGCUGAGGACUUUGUGAGUGGGCCAAACACUUCUGGUCUUGAAACCAGAGUUGUCCAGGCAGAACGCUUCUUCAAAACCCACCUCUUCGAUUGGGUCACCAGUGACCCAUUCGGACGCUGCCACCUGUUGGAACUCCCAACUGACCACAAAGGUGUCUACGGAAGUCUGACCGACUCGUACGCAUAUAUGAGAAACGGUUGGGACGUUGAAGUCACCGCGGUUGGCAACCAGUUCAACGGAGGGUGUCUGUUGGUGGCGAUGGUGCCAGAGCUUUGUUCCAUCCAGAAGAGGGAGCUGUACCAGCUCACGCUCUUCCCCCAUCAGUUCAUCAACCCACGCACAAACAUGACUGCGCACAUCACUGUGCCUUUCGUUGGCGUCAAUCGCUACGACCAGUACAAGGUGCACAAACCUUGGACCCUCGUGGUUAUGGUCGUAGCCCCCCUGACUGUCAACACUGAAGGCGCUCCACAGAUCAAGGUGUACGCCAACAUUGCCCCAACCAACGUGUACGUAGCGGGCGAGUUUCCUUCCAAAGAGGGAAUUUUCCCCGUAGCUUGCAGUGACGGCUAUGGUGGCCUGGUGACCACGGACCCGAAGACGGCUGACCCCGUUUACGGGAAAGUGUUUAACCCCCCACGCAACCUGCUGCCUGGGCGUUUUACCAAUCUCCUUGAUGUGGCUGAGGCAUGCCCGACGUUUCUGCACUUCGAGGGUGACGUGCCAUACGUGACCACGAAAACAGAUUCAGACAGGGUGCUUGCCCAGUUUGACGUGUCCUUAGCAGCAAAGCACAUGUCGAACACCUUUCUUGCAGGUCUCGCGCAGUACUAUGCACAGUACAGUGGCACCAUCAACCUGCACUUCAUGUUUACAGGACCCACUGACGCGAAAGCGCGUUACAUGGUUGCUUACGCCCCACCGGGCAUGGAGCCGCCCCGGACGCCUGAGGCGGCUGCACACUGCAUUCAUGCUGAGUGGGACACUGGGCUAAACUCAAAGUUCACUUUCUCCAUCCCCUAUCUUUCGGCUGCUGACUACGCAUACACCGCGUCAGACGUGGCUGAAACCACGAACGUGCAAGGAUGGGUCUGCUUGUUUCAAAUCACGCACGGCAAAGCCGACGGCGACGCUUUGGUCGUGUUGGCUAGCGCUGGCAAGGACUUUGAGCUGAGGCUGCCGGUCGACGCCCGCAGAGAAACUACCUCUGCGGGUGAAUCAGCAGAUCCCGUGACUACCACUGUUGAGAACUACGGUGGUGAGACACAGGUCCAGAGGCGCCAACACACAGACGUCUCGUUCAUCAUGGACAGAUUCGUGAAAGUGACACCGCAAGAUCAAAUCAACGUUUUGGACCUUAUGCAGAUCCCUGCCCACACGCUGGUGGGAGCGCUCCUGCGCGCGUCCACUUACUACUUCUCUGACCUGGAGAUAGCAGUAAAGCACGAGGGAGACCUCACUUGGGUCCCGAACGGGGCGCCUGAAGCAGCGCUGGAGAACACCACCAACCCAACAGCUUACCACAAGGCACCACUCACUCGGCUCGCCCUGCCUUACACGGCACCACACCGCGUGUUGGCAACCGUGUAUAAUGGAGUGUGCAAGUACAGUAAAACUGCUGUGCCCAACGUGAGAGGUGACACCCAGGUGUUGGAUCAGAAGGCGUCGCGGGCGCUACCCACCUCCUUCAACUACGGUGCCAUCAAAGCAACCCGAGUUAACGAGCUACUUUAUCGGAUGAAGAGGGCUGAGACAUACUGUCCCAGGCCCUUGCUGGCUAUCCAUCCAGCCGAAGCCAGACACAAACAGAAAAUUGUGGCACCUGUGAAACAGACUUUGAACUUUGACCUUCUCAAGUUGGCUGGAGACGUGGAGUCCAACCCUGGGCCCUUCUUCUUCUCCGACGUCAGGUCAAAUUUCUCCAAAUUGGUAGAAACCAUCAACCAGAUGCAGGAGGACAUCUCGACAAAACACGGGCCUGACUUUAACCGGUUGGUGUCUGCGUUUGAGGAAUUGGCUACUGGAGUGAAAGCAAUCAGGACCGGUCUCGACGAGGCCAAACCCUGGUACAAGCUUAUUAAGCUCCUAAGCCGCCUGUCGUGUAUGGCCGCUGUGGCAGCACGGUCCAAGGACCCAGUCCUUGUGGCCAUCAUGCUGGCCGACACCGGUCUCGAGAUUCUGGACAGCACCUUUGUCGUGAAGAAAAUUUCCGAUUCGCUCUCCAGUCUCUUUCACGUGCCGGCCCCCGUCUUCAGUUUCGGAGCCCCGAUCCUGUUGGCCGGGUUGGUCAAGGUCGCCUCGAGUUUCUUCCGGUCCACACCCGAAGACCUUGAGAGAGCAGAGAAACAGCUCAAAGCACGUGACAUUAACGACAUUUUCGCCGUUCUCAAGAACGGCGAGUGGCUGGUCAAGUUGAUCCUUGCCAUCCGCGACUGGAUUAAGGCAUGGAUCGCCUCAGAGGAGAAGUUCGUCACCAUGACAGACCUGGUGCCUGGUAUCCUUGAAAAGCAGCGGGACCUCAACGACCCAAGCAAGUACAAGGAAGCCAAGGAGUGGCUCGACAACGCGCGCCAAGCGUGUUUGAAGAACGGGAACGUCCACAUCGCUAACCUGUGCAAAGUGGUCGCCCCAGCACCCAGCGAGUCGAGACCCGAACCCGUGGUCGUUUGCCUCCGUGGCAAGUCCGGCCAGGGCAAGAGUUUCCUUGCGAACGUGCUCGCGCAAGCAAUCUCCACUCACUUUACUGGUAGAACCAAUUCCGUUUGGUACUGCCCACCUGACCCUGACCACUUCGACGGCUACAACCAGCAGACCGUUGUAGUGAUGGAUGACCUGGGUCAGAACCCCGAUGGCAAGGACUUCAAGUACUUCGCCCAAAUGGUUUCAACCACGGGGUUCAUCCCGCCCAUGGCGUCACUCGAGGACAAAGGCAAACCUUUCAACAGCAAGGUCAUCAUCGCAACCACCAACCUGUACUCGGGUUUUACCCCGAAGACUAUGGUGUGCCCUGAUGCGCUGAACCGGAGGUUCCACUUUGACAUCGACGUGAGCGCCAAGGACGGGUACAAAAUUAACAACAAACUGGACAUCGUCAAAGCACUUGAAGACACUCACACCAACCCGGUAGCAAUGUUUCAGUACGACUGUGCCCUUCUCAACGGCACGGCCGUUGAAAUGAAGAGAAUGCAGCAGGACUUGUUCAAGCCUCAACCACCCCUCCAGAACGUGUACCAGCUCGUUCAGGAGGUGAUUGAACGGGUCGAGCUCCACGAGAAAGUGUCGAGCCACCCGAUUUUCAAGCAGAUCUCAAUUCCUUCCCAAAAAUCCGUGUUGUACUUCCUCAUUGAGAAAGGUCAACACGAGGCGGCAAUUGAAUUCUUUGAGGGCAUGGUGCACGACUCCAUCAAGGAGGAGCUCCGACCCCUCAUCCAACGAACCUCAUUUGUGAAACGCGCUUUUAAGCGUCUGAAGGAAAACUUUGAGACUGUUGCCCUGUGCUUGACCCUCUUGGCCAACAUAGUGAUCAUGAUCCGUGAAACCCACAAGAGGCAGAAGAUGGUGGAUGGCUCGGUGAAUGAGCACAUCGAGAAGGCAAACAACACCGCAGACGAUGAAACUCUUGACGAGGCGGGAAAGAACCCUCUGGAAACCAGUGGUGCCAGCACCGUUGGCUUCAGAGAGAGAACUCUCACGGACCGAAAGGCGCGUGAUGACGUGGGCUUCGAGCCCGCAUAUCCUGCUGAAGAGUACCCACAAGCUGAAGGACCCUACGCCGGGCCACUCGAGCGUCAGAGACCUCUGAAAGUGAGAGCCAAGCUCCCACAACAGGAGGGACCCUACGCUGGUCCGAUGGAGAGACAGAAACCACUGAAAGUGAAAGUGAAAAACCCGGUCAUUAAGGAAGGACCCUACGAAGGACCGGUGAAGAAGCCUGUCGCUUUGAAAGUGAAAGCCAAGAACUUAAUUGUCACUGAGAGUGGUGCCCCACCGACCGACUUGCAAAAGAUGGUCAUGGGCAACACAAAGCCUGUUGAGCUUAUCCUUGACGGGAAGACAGUAGCCAUCUGCUGUGCUACUGGAGUGUUUGGUACUGCUUACCUCGUGCCUCGUCACCUUUUUGCAGAGAAGUAUGACAAAAUCAUGUUGGACGGCAGAGCCAUGACAGACAGUGACUACAGAGUGUUUGAGUUUGAGAUUAAAGUAAAAGGACAGGACAUGCUCUCGGACGCCGCACUCAUGGUACUCCACCGCGGGAACCGUGUGAGAGACAUCACGAAACACUUUCGUGAUACAGCAAGAAUGAAGAAAGGCACUCCCGUUGUUGGUGUGAUCAACAACGCAGACGUCGGGAGGCUGAUUUUCUCUGGUGAGGCCCUCACCUACAAGGAUAUCGUAGUGUGCAUGGAUGGAGAUACCAUGCCUGGCCUUUUUGCCUAUAAGGCCGCCACCAAGGCCGGUUACUGUGGAGGAGCCGUUCUCGCUAAGGACGGGGCUGACACGUUCAUCGUCGGCACUCACUCUGCAGGAGGCAAUGGAGUAGGAUACUGUUCGUGUGUUUCCAGGUCCAUGCUUCAAAAGAUGAAGGCACACAUUGACCCCGAACCACACCACGAGGGUUUGGUUGUCAACACCAGAGAUGUGGAAGAGCGCGUUCACGUGAUGCGCAAAACCAAGCUUGCACCCACCGUUGCACACGGUGUGUUCAACCCUGACUUUGGGCCUGCCGCCUUGUCUAACAAGGACCCGCGCCUGAACGAGGGUGUUGUCCUCGAUGAAGCCAUCUUCUCCAAACACAAGGGAGACACAAAGAUGUCCGAGGAAGACAAAGCGCUGUUCCGCCGUUGUGCUGCUGACUACGCCUCACGCUUGCACAGCGUACUGGGUACAGCAAAUGCCCCAUUGAGCAUCUACGAGGCAAUCAAGGGCGUUGAUGGACUCGACGCCAUGGAGCCAGACACUGCGCCUGGCCUCCCCUGGGCACUCCAGGGGAAACGUCGUGGCGCGCUCAUCGAUUUUGAGAACGGCACGGUCGGGCCCGAAGUCGAGGCUGCCUUGAAGCUCAUGGAGAGAAGAGAAUACAAAUUUGUUUGCCAGACCUUCCUGAAGGACGAAAUUCGCCCGAUGGAGAAAGUACGUGCCGGCAAGACUCGCAUUGUCGACGUUUUGCCCGUUGAACACAUUCUUUACACCAGGAUGAUGAUUGGCAGAUUCUGUGCACAAAUGCACUCAAACAACGGACCGCAAAUUGGCUCAGCAGUCGGUUGUAACCCUGAUGUUGAUUGGCAAAGAUUUGGCACACACUUCGCCCAAUACAGAAACGUGUGGGACGUGGACUACUCGGCCUUCGAUGCUAACCACUGCAGUGACGCAAUGAACAUCAUGUUUGAGGAAGUGUUCCGCACGGACUUUGGUUUUCACCCAAAUGCUGAGUGGAUCCUGAAGACUCUCGUGAACACGGAACACGCCUAUGAAAAUAAACGUAUCACUGUCGAGGGCGGGAUGCCAUCUGGUUGUUCCGCAACAAGCAUCAUCAACACAAUCUUGAACAACAUAUAUGUGCUCUACGCCUUGCGUAGACACUAUGAGGGAGUUGAGCUGGAUACUUACACCAUGAUCUCAUACGGAGACGACAUCGUGGUGGCAAGUGACUACGAUUUGGACUUUGAGGCUCUCAAGCCCCACUUUAAAUCUCUCGGUCAAACCAUUACUCCAGCUGACAAAAGCGACAAAGGUUUUGUUCUCGGACAUUCCAUCACCGAUGUCACUUUCCUCAAAAGACACUUCCACAUGGAUUAUGGAACUGGGUUUUACAAACCUGUGAUGGCCUCAAAGACUCUCGAGGCCAUUCUCUCCUUUGCACGCCGUGGGACCAUACAGGAGAAGUUGAUCUCCGUGGCAGGACUCGCCGUCCACUCCGGACCAGACGAGUACCGGCGUCUCUUUGAACCCUUCCAGGGCCUCUUUGAGAUUCCAAGCUACAGAUCACUUUACCUGCGUUGGGUGAACGCCGUGUGCGGUGACGCAUAAUCCCUCAGAGAUCACAAUUGGCACAAUGACUCUGGGGCGCGCGACGCCGUAGGAGUGAAAAGCCCGAAGGGCUUCUCCCGCUUCCUUAUCCCAAAAAAAAAAAAAAAA
Lig328 RNA CCCCUCUCCCUCCCCCCCCCCUAACGUUACUGGCCGAAGCCGCUUGGAAUAAGGCCGGUGUGCGUUUGUCUAUAUGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGAAACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGC