P26599_RRM2

Protein information

  • Protein ID: P26599
  • Protein Name: Polypyrimidine tract-binding protein 1
  • Organism: Homo sapiens
  • Gene Name: PTBP1

Domain information

  • Pfam ID: PF00076
  • Pfam Clan Name: RRM
  • Domain Range: 186 - 252
  • Domain Length: 67

Domain Sequence

  • IIVENLFYPVTLDVLHQIFSKFGTVLKIITFTKNNQFQALLQYADPVSAQHAKLSLDGQNIYNACCT

Available Structures for this domain - 6 

Experiment ID RRM Structure Experiment Type Ligand ID Contacts
Exp_1SJR 1SJRA01 NMR No contacts
Exp_2ADB 2ADBA01 NMR Lig223 Contacts
Exp_3ZZY 3ZZYB01 X-ray Diffraction No contacts
Exp_3ZZY 3ZZYA01 X-ray Diffraction No contacts
Exp_3ZZZ 3ZZZB01 X-ray Diffraction No contacts
Exp_3ZZZ 3ZZZA01 X-ray Diffraction No contacts

Binding Experiments for this domain - 3 

Experiment ID RRM Entryname Experiment Type Ligand ID Binds? Binding Affinity
Exp_253 P26599_RRM2 Filter binding assay Lig328 Yes 0.026 ± μM
Exp_259 P26599_RRM2 Filter binding assay Lig232 Yes 0.038 ± μM
Exp_262 P26599_RRM2 Filter binding assay Lig301 Yes 0.061 ± μM

Ligands tested for Binding - 4 

Ligand ID Ligand Type Ligand Sequence
Lig223 RNA CUCUCU
Lig232 RNA UUGAAAGCCGGGGGUGGGAGAUCCGGAUUGCCAGUCUGCUCGAUAUCGCAGGCUGGGUCCGUGACUACCCACUCCCCCUUUCAACGUGAAGGCUACGAUAGUGCCAGGGCGGGUACUGCCGUAAGUGCCACCCCAAAAUAACAACAGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCUCUCCCUCCCCCCCCCCUAACGUUACUGGCCGAAGCCGCUUGGAAUAAGGCCGGUGUGCGUUUGUCUAUAUGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGAAACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGCAAGGUCUGUUGAAUGUCGUGAAGGAAGCAGUUCCUCUGGAAGCUUCUUGAAGACAAACAACGUCUGUAGCGACCCUUUGCAGGCAGCGGAACCCCCCACCUGGCGACAGGUGCCUCUGCGGCCAAAAGCCACGUGUAUAAGAUACACCUGCAAAGGCGGCACAACCCCAGUGCCACGUUGUGAGUUGGAUAGUUGUGGAAAGAGUCAAAUGGCUCUCCUCAAGCGUAUUCAACAAGGGGCUGAAGGAUGCCCAGAAGGUACCCCAUUGUAUGGGAUCUGAUCUGGGGCCUCGGUGCACAUGCUUUACAUGUGUUUAGUCGAGGUUAAAAAACGUCUAGGCCCCCCGAACCACGGGGACGUGGUUUUCCUUUGAAAAACACGAUGAUAAUAUGGCCACAACCAUGGAACAAGAGACUUGCGCGCACUCUCUCACUUUUGAGGAAUGCCCAAAAUGCUCUGCUCUACAAUACCGUAAUGGAUUUUACCUGCUAAAGUAUGAUGAAGAAUGGUACCCAGAGGAGUUAUUGACUGAUGGAGAGGAUGAUGUCUUUGAUCCCGAAUUAGACAUGGAAGUCGUUUUCGAGUUACAGGGCAAUUCCACCUCCUCAGACAAGAAUAACUCCUCCUCGGAAGGCAAUGAAGGUGUGAUCAUCAAUAACUUUUACUCCAACCAAUAUCAAAACUCCAUUGACCUCUCUGCUAAUGCAGCCGGGUCUGACCCACCCAGAACCUACGGUCAAUUUUCGAAUCUCUUUUCGGGCGCAGUGAAUGCCUUUUCUAAUAUGCUUCCAUUGCUAGCUGAUCAAAAUACAGAAGAAAUGGAGAAUCUGUCUGAUCGAGUGUCUCAAGACACUGCCGGCAAUACGGUCACAAACACCCAGUCAACAGUGGGCCGUCUUGUCGGUUAUGGUACCGUUCAUGAUGGAGAGCAUCCGGCAUCAUGUGCUGACACUGCUUCAGAAAAGAUCCUGGCGGUGGAAAGGUACUACACCUUCAAGGUUAAUGAUUGGACAUCAACACAAAAGCCCUUUGAGUACAUCCGCAUUCCCCUUCCUCACGUCCUGUCCGGUGAAGAUGGUGGUGUCUUUGGUGCGGCCCUCCGCCGGCACUACCUGGUGAAAACUGGAUGGCGGGUGCAAGUUCAGUGCAACGCCUCUCAAUUCCACGCUGGAGGUUUGCUGGUGUUCAUGGCACCAGAGUAUCCAACCUUAGAUGCUUUUGCCAUGGACAACCGUUGGUCCAAGGAUAACCUGCCUAAUGGAACCAGAACUCAGACAAACAAAAAGGGACCAUUUGCCAUGGACCAUCAGAACUUCUGGCAGUGGACCUUGUAUCCCCAUCAAUUCCUGAAUCUGAGAACUAACACCACAGUGGAUCUUGAGGUGCCAUAUGUAAACAUAGCCCCCACUUCCUCCUGGACACAACAUGCUUCCUGGACUUUGGUGAUUGCAGUGGUUGCUCCCCUGACAUACUCAACCGGGGCUUCUACCAGUUUGGAUAUCACCGCUUCUAUUCAGCCAGUAAGGCCUGUCUUUAAUGGCCUCCGGCAUGAGACACUUUCUAGACAGUCGCCCAUUCCGGUCACAAUUAGAGAACAUGCUGGUACCUGGUAUUCUACUCUGCCAGACAGCACAGUGCCUAUUUAUGGCAAGACUCCUGUUGCUCCAUCCAAUUACAUGGUAGGCGAAUACAAGGACUUCCUGGAGAUAGCUCAGAUUCCAACCUUUAUUGGGAAUAAGAUCCCUAAUGCUGUCCCCUACAUUGAGGCAUCCAACACAGCCGUCAAGACCCAACCGCUGGCCACCUAUCAAGUGACCUUGUCCUGCUCCUGUCUGGCCAAUACAUUCUUGGCCGCUUUGUCUAGAAACUUUGCUCAGUACCGGGGAUCAUUGGUUUAUACCUUUGUGUUCACUGGGACCGCGAUGAUGAAGGGCAAGUUCCUCAUUGCCUACACCCCACCUGGAGCGGGCAAGCCCACUAGUCGAGACCAAGCCAUGCAGGCGACUUAUGCGAUUUGGGAUUUGGGGCUAAAUUCUUCUUACUCCUUCACUGUGCCUUUUAUUUCUCCCACUCACUUCCGCAUGGUAGGUACUGACCAAGUCAACAUCACUAAUGCGGAUGGCUGGGUUACCGUGUGGCAGCUCACUCCCCUCACUUACCCACCAGGAUGCCCGACCUCUGCUAAGAUACUAACAAUGGUGAGCGCAGGGAAGGAUUUCUCACUCAAGAUGCCUAUCUCACCUGCCCCCUGGAGCCCUCAGGGAGUAGAAAACGCUGAAAAAGGGGUCACUGAAAACACAAACGCAACUGCUGACUUUGUGGCUCAACCAGUUUACUUGCCUGAGAACCAAACGAAGGUGGCUUUCUUCUAUAAUAGGUCCAGUCCCAUUGGUGCCUUCACCGUGAAGUCCGGCAGUCUAGAAUCUGGUUUUGCCCCGUUCUCUAAUGGGACUUGCCCGAACUCAGUGAUACUGACCCCUGGGCCCCAAUUUGACCCCGCCUAUGACCAACUCAGGCCACAGCGUCUGACAGAAAUUUGGGGCAAUGGAAAUGAGGAGACCUCAAAAGUCUUUCCGCUUAAAUCCAAACAGGAUUAUUCCUUCUGCCUCUUCUCCCCCUUUGUGUAUUAUAAAUGUGAUUUAGAAGUGACUCUUAGUCCUCACACUUCAGGCAACCAUGGGCUGUUGGUGAGGUGGUGUCCCACUGGUACACCAACCAAGCCCACUACCCAGGUUCUCCAUGAAGUAAGUUCCCUCUCAGAAGGCAGAACCCCCCAGGUUUAUAGUGCCGGACCUGGCAUUUCAAAUCAGAUUUCCUUUGUAGUUCCUUACAAUUCUCCACUUUCAGUCCUAUCAGCUGUCUGGUAUAAUGGACACAAGAGAUUUGACAACACUGGGAGCUUGGGCAUUGCCCCUAAUUCUGAUUUCGGCACUCUGUUCUUUGCUGGCACAAAGCCUGACAUUAAAUUCACAGUCUACUUGAGAUACAAGAAUAAGAGAGUUUUUUGCCCACGUCCGACUGUCUUUUUCCCCUGGCCCACUUCCGGAGACAAGAUUGAUAUGACCCCGAGAGCUGGAGUCUUGAUGCUAGAGAGUCCAAAUGCCCUAGACAUUUCAAGAACAUACCCCACGUUACAUGUUCUCAUUCAAUUCAACCAUAGAGGUUUGGAGGUUAGAUUGUUUAGACAUGGACACUUUUGGGCUGAAACACGUGCGGACGUGAUUCUGAGAUCAAAGACCAAACAGGUCUCUUUCCUGAGCAACGGGAACUACCCGUCAAUGGACUCUAGAGCUCCCUGGAAUCCUUGGAAGAAUACCUACCAGGCGGUUCUAAGAGCAGAACCAUGUAGAGUGACCAUGGAUAUAUAUUAUAAGAGAGUCAGGCCUUUUAGACUGCCCCUGGUUCAGAAGGAAUGGCCCGUGCGAGAGGAGAACGUUUUCGGUUUGUACCGGAUCUUCAAUGCCCACUACGCUGGUUACUUUGCGGACCUACUGAUUCAUGACAUUGAGACAAAUCCAGGGCCCUUCAUGUUUAGACCAAGGAAACAGGUUUUCCAGACCCAAGGAGCGGCAGUGUCAUCAAUGGCUCAAACCCUACUGCCGAACGACCUUGCCAGCAAAGCUAUGGGAUCAGCUUUUACGGCUUUGCUCGAUGCCAACGAGGACGCCCAAAAAGCAAUGAAGAUUAUAAAGACAUUAAGUUCUCUAUCGGAUGCAUGGGAAAAUGUAAAAGAAACACUAAACAACCCAGAGUUCUGGAAGCAGCUCUUGAGCAGAUGUGUGCAGCUGAUUGCAGGGAUGACAAUAGCAGUGAUGCAUCCGGACCCUUUGACUCUGCUCUGCUUAGGAACAUUGACGGCCGCCGAGAUUACAAGCCAGACAAGUCUGUGCGAAGAAAUAGCAGCUAAGUUCAAGACAAUUUUCAUCACUCCUCCACCACGGUUUCCCACAAUCUCUCUUUUCCAACAACAAUCCCCCUUGAAACAGGUAAAUGAUAUUUUCUCCCUAGCCAAGAACCUGGACUGGGCCGUCAAGACUGUGGAAAAGGUGGUUGAUUGGUUUGGGACAUGGAUAGUACAGGAGGAAAAGGAACAGACCCUAGAUCAGCUCUUGCAGCGUUUCCCCGAACAUGCGAAGCGCAUUUCUGAUCUCCGGAAUGGAAUGGCCGCCUAUGUAGAGUGCAAGGAGAGUUUUGAUUUCUUUGAAAAGCUGUACAAUCAGGCAGUGAAAGAGAAGAGAACGGGUAUCGCCGCCGUCUGUGAAAAAUUCAGACAGAAGCAUGACCACGCCACCGCUCGGUGUGAGCCAGUCGUGAUUGUGCUCCGCGGAGACGCGGGGCAAGGGAAAUCUUUAUCAAGUCAGGUUAUUGCCCAGGCCGUCUCCAAGACCAUUUUCGGCCGGCAAUCUGUGUAUUCCCUUCCCCCCGAUUCGGAUUUCUUUGAUGGCUAUGAAAAUCAGUUUGCAGCAAUAAUGGAUGAUCUAGGGCAAAAUCCUGAUGGCUCUGAUUUCACUACGUUCUGUCAGAUGGUUUCGACUACCAAUUUUCUCCCCAAUAUGGCUAGUCUAGAGAGAAAGGGCACCCCCUUUACAUCUCAGCUUGUGGUGGCAACUACCAAUCUGCCUGAGUUUAGACCUGUCACAAUAGCCCAUUACCCUGCUGUUGAGAGAAGGAUAACUUUCGACUAUUCAGUGUCUGCUGGUCCAGUCUGCUCCAAAACAGAGGCCGGGUAUAAGGUUUUGGAUGUUGAAAGAGCCUUUAGGCCUACCGGUGAGGCUCCUCUUCCAUGCUUCCAGAAUAACUGCCUUUUCCUUGAGAAAGCUGGGCUCCAGUUCAGAGAUAACCGAACUAAAGAGAUCAUUUCCCUGGUAGAUGUGAUUGAGAGAGCCGUGGCUAGGAUUGAAAGGAAGAAGAAAGUUCUCACAACCGUGCAGACCCUUGUGGCACAAGGUCCAGUAGACGAGGUCAGUUUCCAUUCCGUAGUCCAGCAGCUUAAAGCAAGACAGCAAGCGACAGAUGAACAGCUUGAGGAAUUGCAAGAGGCUUUCGCGAAAGUACAGGAGCGUAACUCUGUGUUUUCUGAUUGGUUGAAGAUUUCUGCAAUGUUGUGUGCUGCGACUCUGGCACUUUCCCAAGUUGUCAAGAUGGCCAAGGCGGUGAAGCAGAUGGUCAAGCCUGAUCUGGUUCGUGUGCAAUUGGAUGAGCAGGAGCAGGGACCUUACAAUGAGACAGCGAGAGUUAAACCAAAAACACUGCAGUUGUUGGACAUUCAGGGACCAAACCCUGUGAUGGACUUUGAAAAAUAUGUAGCCAAACAUGUAACCGCCCCCAUUGGUUUUGUCUACCCCACUGGGGUGAGCACCCAGACUUGCCUCCUUGUGAGAGGCCGCACCUUGGUAGUAAAUAGACACAUGGCCGAGUCUGACUGGACUUCCAUAGUAGUGCGUGGAGUCACACACGCCCGCUCUACUGUUAAAAUUUUGGCCAUAGCUAAAGCAGGCAAAGAGACUGACGUAUCUUUCAUCCGCCUCUCUUCUGGUCCUCUAUUCAGAGACAAUACAUCCAAAUUUGUCAAGGCUGGUGAUGUACUUCCUACUGGUGCCGCUCCAGUCACGGGGAUAAUGAACACGGACAUACCCAUGAUGUACACAGGAACCUUCCUGAAAGCUGGUGUGUCAGUCCCAGUGGAAACCGGCCAGACCUUUAAUCACUGUAUUCAUUACAAGGCUAACACACGAAAGGGCUGGUGUGGAUCAGCCCUACUGGCAGAUCUUGGAGGAAGCAAGAAAAUCCUUGGCAUCCAUUCUGCUGGCUCUAUGGGAAUAGCCGCCGCCUCGAUUGUGUCACAGGAGAUGAUUCGGGCGGUAGUGAAUGCCUUUGAGCCACAGGGUGCUCUCGAGAGAUUGCCAGAUGGGCCCCGUAUUCACGUACCACGUAAAACAGCACUACGCCCCACCGUUGCCCGUCAAGUCUUCCAACCAGCAUAUGCCCCGGCUGUUCUAUCGAAAUUUGACCCUAGAACAGAGGCUGAUGUAGAUGAAGUGGCUUUCUCCAAACAUACCUCCAACCAGGAAAGCCUCCCACCAGUGUUUAGAAUGGUAGCCAAAGAGUAUGCCAAUAGAGUUUUCACCUUGCUGGGAAAAGACAAUGGCCGUCUGACUGUAAAGCAGGCUUUGGAAGGACUGGAGGGGAUGGACCCCAUGGACAGGAACACCUCCCCGGGGCUUCCAUAUACUGCGCUAGGAAUGCGCAGAACAGAUGUCGUAGAUUGGGAAUCAGCCACCCUGAUCCCGUUUGCGGCAGAAAGAUUAAGAAAAAUGAAUGAAGGAGACUUUUCCGAAGUUGUCUAUCAAACAUUCCUCAAGGAUGAGCUUAGACCGAUAGAGAAGGUUCAAGCCGCCAAGACACGGAUUGUAGAUGUUCCACCAUUUGAGCAUUGCAUUCUGGGUAGACAAUUGUUGGGAAAGUUUGCAUCAAAGUUCCAGACCCAACCGGGUCUGGAACUAGGAUCAGCCAUUGGAUGUGACCCAGAUGUACACUGGACUGCCUUCGGUGUCGCCAUGCAAGGUUUUGAGCGUGUCUACGAUGUGGACUACUCCAACUUUGAUUCGACCCAUUCGGUGGCAAUGUUCCGCUUAUUGGCUGAGGAAUUUUUCACUCCAGAGAAUGGUUUUGACCCCCUGACUAGAGAAUAUCUUGAGUCAUUAGCCAUUUCAACCCAUGCGUUUGAGGAGAAGCGCUUUCUGAUAACCGGUGGUCUCCCAUCAGGUUGUGCAGCGACCUCAAUGCUAAACACUAUAAUGAAUAAUAUAAUAAUUAGGGCGGGUUUGUAUCUCACGUAUAAAAAUUUUGAAUUUGAUGAUGUGAAGGUGUUGUCGUACGGAGAUGAUCUCCUUGUGGCCACAAAUUACCAAUUGGAUUUUGAUAAGGUGAGAGCAAGCCUCGCAAAGACAGGAUAUAAGAUAACUCCCGCUAACACAACUUCUACCUUUCCUCUUAAUUCGACGCUUGAAGACGUUGUCUUCUUAAAAAGAAAGUUUAAGAAAGAGGGCCCUCUGUAUCGGCCUGUCAUGAACAGAGAGGCGUUGGAAGCAAUGUUGUCAUACUAUCGUCCAGGGACUCUAUCUGAGAAACUCACUUCGAUCACUAUGCUUGCCGUUCAUUCUGGCAAGCAGGAAUAUGAUCGGCUCUUUGCCCCAUUCCGUGAGGUAGGGGUUGUCGUGCCAUCAUUCGAGAGUGUGGAGUACAGAUGGAGGAGUCUGUUCUGGUAGUAGUGUAGUCACUGGCACAACGCGUUACCCGGUAAGCCAAUCGGGUAUACACGGUCGUCAUACUGCAGACAGGGUUCUUCUACUUUGCAAGAUAGUCUAGAGUAGUAAAAUAAAUAGAUAGAG
Lig301 RNA UUGAAAGGGGGCGCUAGGGUUUCACCCCUAGCACGCCAACGACAGUCCCUGCGUUGCACUCCACACUUACGUUCGCGUGCACGCGGGACCCGAUGGACUUUCGUUCACCCACCUACGGUUGGACUCACGGCACCGCGUGGCCAUUUUAGCUGGACCGUGUGGACGAACACCGCUUGCGCUUCUCGCGUGACCGGCUAGUACUCUGACCACUCUCCGCCUACUUGGUCGCUGCGCUGUCUUGGGCACUCCUAUUGGGGUCGUUCGACGCUCCACGGGCUCCCCCGUGCGGCGGGCUACGGUGAUGGGGCCGUUUCGCGCGGGCUGAUCGCCUGGUCUGCUUCGGCUGUCACCCGAAGCCCGCCUUUCACCCCCCCCCCCCUAAGUUUUACCGUCGUUUCCGACGUAAAGGGGAAGUAACCACAAGCUUGACACCGCCUGUCCCGGCGUUAAAGGGAAGUAACCACAAGCUUACAACCGCCUACCCCGGUGUUAAUGGGAUGUAACCACAAGAUACACCUUCACCCGGAAGUAAAACGGCAAAUUCACACAGUUUUGCCCGUUUUUCAUGAGAAACGGGACGUCUGCGCACGAAACGCGCCGUCGCUUGAGGAGGACUUGUACAAACACGAUCUAAACAGGUUUCCCCAACUGACAUACACCGUGCAAUUUGAAACUCCGCCUGGUCUUUCCAGGUCUAGAGGGGUAACACUUUGUACUGUGCUUGACUCCACGCUCGGUCCACUGGCGAGUGUUAGUAACAGCACUGGUGCUUCGUAGCGGAGCAUGGUGGCCGUGGGAACUCCUCCUUGGUAACAAGGACCCACGGGGCCGAAAGCCACGUCCUGACGGACCCACCAUGUGUGCAACCCCAGCACGGCAACUUUUCUGUGAAACUCACUCUAAGGUGACACUGAUACUGGUAUUCAAGUACUGGUGACAGGCUAAGGAUGCCCUUCAGGUACCCCGAGGUAACACGCGACACUCGGGAUCUGAGAAGGGGACUGGGGCUUCUGUAAAAGCGCCCAGUUUAAAAAGCUUCUAUGCCUGGAUAGGUGACCGGAGGCCGGCGCCUUUCCAUUAUAACUACUGACUUUAUGAACACAACUAACUGUUUUAUCGCUUUGGUGUACGCUAUCAGAGAGAUCAGAACGCUUUUCCUUUCACGAGCUACAGGAAAGAUGGAAUUCACGCUGUACAACGGUGAAAAGAAGACCUUUUACUCCAGGCCCAACAACCACGACAACUGUUGGUUGAACACCAUCCUUCAGUUGUUCAGGUACGUCGAUGAGCCUUUCUUCGACUGGGUCUACAACUCACCCCAGAACCUCACGCUUGAAGCCAUCAAACAGCUGGAAGAACUCACAGGGCUUGAGUUACACGAAGGCGGACCGCCUGCCGCUGUUGUUUGGAACAUCAAACACUUGCUUCAAACCGGUAUUGGUACCGCCUCGCGACCUAGCGAGGUGUGUGUGGUGGACGGUACGAACAUGUGUCUGGCUGAUUUCCAUGCAGGCAUCUUCCUGAAAGGACAGGAACAUGCCGUGUUUGCUUGUGUCACCUCCAACGGGUGGUACGCGAUUGACGAUGAGGACUUCUACCCAUGGACGCCGGACCCGUCCGACGUUCUGGUGUUCGUUCCAUAUGAUCAAGAACCACUCAACGGAGAAUGGAAAAUCAACGUUCAGCGGAAGCUCAAGGGAGCUGGACAAUCCAGUCCGGCGACCGGCUCGCAGAACCAAUCUGGCAAUACUGGCAGCAUAAUCAACAACUACUACAUGCAACAGUACCAGAACUCCAUGGACACACAACUUGGUGACAACGCCAUCAGCGGAGGCUCCAACGAGGGCUCCACAGACACAACUUCCAAUCACACAACCAACACUCAGAACAACGACUGGUUUUCCAAGCUCGCAGGUUCUGCCUUCAGCGGUCUUUUUGGCGCCCUUCUUGCUGACAAAAAGACAGAGGAAACCACUCUCCUCGAGGACCGCAUCCUCACUACCCGCAACGGACACACGACCUCAACAACACAGUCGAGUGUCGGAGUCACGUACGGGUAUGCAACAGCUGAGGACUUUGUGAGUGGGCCAAACACUUCUGGUCUUGAAACCAGAGUUGUCCAGGCAGAACGCUUCUUCAAAACCCACCUCUUCGAUUGGGUCACCAGUGACCCAUUCGGACGCUGCCACCUGUUGGAACUCCCAACUGACCACAAAGGUGUCUACGGAAGUCUGACCGACUCGUACGCAUAUAUGAGAAACGGUUGGGACGUUGAAGUCACCGCGGUUGGCAACCAGUUCAACGGAGGGUGUCUGUUGGUGGCGAUGGUGCCAGAGCUUUGUUCCAUCCAGAAGAGGGAGCUGUACCAGCUCACGCUCUUCCCCCAUCAGUUCAUCAACCCACGCACAAACAUGACUGCGCACAUCACUGUGCCUUUCGUUGGCGUCAAUCGCUACGACCAGUACAAGGUGCACAAACCUUGGACCCUCGUGGUUAUGGUCGUAGCCCCCCUGACUGUCAACACUGAAGGCGCUCCACAGAUCAAGGUGUACGCCAACAUUGCCCCAACCAACGUGUACGUAGCGGGCGAGUUUCCUUCCAAAGAGGGAAUUUUCCCCGUAGCUUGCAGUGACGGCUAUGGUGGCCUGGUGACCACGGACCCGAAGACGGCUGACCCCGUUUACGGGAAAGUGUUUAACCCCCCACGCAACCUGCUGCCUGGGCGUUUUACCAAUCUCCUUGAUGUGGCUGAGGCAUGCCCGACGUUUCUGCACUUCGAGGGUGACGUGCCAUACGUGACCACGAAAACAGAUUCAGACAGGGUGCUUGCCCAGUUUGACGUGUCCUUAGCAGCAAAGCACAUGUCGAACACCUUUCUUGCAGGUCUCGCGCAGUACUAUGCACAGUACAGUGGCACCAUCAACCUGCACUUCAUGUUUACAGGACCCACUGACGCGAAAGCGCGUUACAUGGUUGCUUACGCCCCACCGGGCAUGGAGCCGCCCCGGACGCCUGAGGCGGCUGCACACUGCAUUCAUGCUGAGUGGGACACUGGGCUAAACUCAAAGUUCACUUUCUCCAUCCCCUAUCUUUCGGCUGCUGACUACGCAUACACCGCGUCAGACGUGGCUGAAACCACGAACGUGCAAGGAUGGGUCUGCUUGUUUCAAAUCACGCACGGCAAAGCCGACGGCGACGCUUUGGUCGUGUUGGCUAGCGCUGGCAAGGACUUUGAGCUGAGGCUGCCGGUCGACGCCCGCAGAGAAACUACCUCUGCGGGUGAAUCAGCAGAUCCCGUGACUACCACUGUUGAGAACUACGGUGGUGAGACACAGGUCCAGAGGCGCCAACACACAGACGUCUCGUUCAUCAUGGACAGAUUCGUGAAAGUGACACCGCAAGAUCAAAUCAACGUUUUGGACCUUAUGCAGAUCCCUGCCCACACGCUGGUGGGAGCGCUCCUGCGCGCGUCCACUUACUACUUCUCUGACCUGGAGAUAGCAGUAAAGCACGAGGGAGACCUCACUUGGGUCCCGAACGGGGCGCCUGAAGCAGCGCUGGAGAACACCACCAACCCAACAGCUUACCACAAGGCACCACUCACUCGGCUCGCCCUGCCUUACACGGCACCACACCGCGUGUUGGCAACCGUGUAUAAUGGAGUGUGCAAGUACAGUAAAACUGCUGUGCCCAACGUGAGAGGUGACACCCAGGUGUUGGAUCAGAAGGCGUCGCGGGCGCUACCCACCUCCUUCAACUACGGUGCCAUCAAAGCAACCCGAGUUAACGAGCUACUUUAUCGGAUGAAGAGGGCUGAGACAUACUGUCCCAGGCCCUUGCUGGCUAUCCAUCCAGCCGAAGCCAGACACAAACAGAAAAUUGUGGCACCUGUGAAACAGACUUUGAACUUUGACCUUCUCAAGUUGGCUGGAGACGUGGAGUCCAACCCUGGGCCCUUCUUCUUCUCCGACGUCAGGUCAAAUUUCUCCAAAUUGGUAGAAACCAUCAACCAGAUGCAGGAGGACAUCUCGACAAAACACGGGCCUGACUUUAACCGGUUGGUGUCUGCGUUUGAGGAAUUGGCUACUGGAGUGAAAGCAAUCAGGACCGGUCUCGACGAGGCCAAACCCUGGUACAAGCUUAUUAAGCUCCUAAGCCGCCUGUCGUGUAUGGCCGCUGUGGCAGCACGGUCCAAGGACCCAGUCCUUGUGGCCAUCAUGCUGGCCGACACCGGUCUCGAGAUUCUGGACAGCACCUUUGUCGUGAAGAAAAUUUCCGAUUCGCUCUCCAGUCUCUUUCACGUGCCGGCCCCCGUCUUCAGUUUCGGAGCCCCGAUCCUGUUGGCCGGGUUGGUCAAGGUCGCCUCGAGUUUCUUCCGGUCCACACCCGAAGACCUUGAGAGAGCAGAGAAACAGCUCAAAGCACGUGACAUUAACGACAUUUUCGCCGUUCUCAAGAACGGCGAGUGGCUGGUCAAGUUGAUCCUUGCCAUCCGCGACUGGAUUAAGGCAUGGAUCGCCUCAGAGGAGAAGUUCGUCACCAUGACAGACCUGGUGCCUGGUAUCCUUGAAAAGCAGCGGGACCUCAACGACCCAAGCAAGUACAAGGAAGCCAAGGAGUGGCUCGACAACGCGCGCCAAGCGUGUUUGAAGAACGGGAACGUCCACAUCGCUAACCUGUGCAAAGUGGUCGCCCCAGCACCCAGCGAGUCGAGACCCGAACCCGUGGUCGUUUGCCUCCGUGGCAAGUCCGGCCAGGGCAAGAGUUUCCUUGCGAACGUGCUCGCGCAAGCAAUCUCCACUCACUUUACUGGUAGAACCAAUUCCGUUUGGUACUGCCCACCUGACCCUGACCACUUCGACGGCUACAACCAGCAGACCGUUGUAGUGAUGGAUGACCUGGGUCAGAACCCCGAUGGCAAGGACUUCAAGUACUUCGCCCAAAUGGUUUCAACCACGGGGUUCAUCCCGCCCAUGGCGUCACUCGAGGACAAAGGCAAACCUUUCAACAGCAAGGUCAUCAUCGCAACCACCAACCUGUACUCGGGUUUUACCCCGAAGACUAUGGUGUGCCCUGAUGCGCUGAACCGGAGGUUCCACUUUGACAUCGACGUGAGCGCCAAGGACGGGUACAAAAUUAACAACAAACUGGACAUCGUCAAAGCACUUGAAGACACUCACACCAACCCGGUAGCAAUGUUUCAGUACGACUGUGCCCUUCUCAACGGCACGGCCGUUGAAAUGAAGAGAAUGCAGCAGGACUUGUUCAAGCCUCAACCACCCCUCCAGAACGUGUACCAGCUCGUUCAGGAGGUGAUUGAACGGGUCGAGCUCCACGAGAAAGUGUCGAGCCACCCGAUUUUCAAGCAGAUCUCAAUUCCUUCCCAAAAAUCCGUGUUGUACUUCCUCAUUGAGAAAGGUCAACACGAGGCGGCAAUUGAAUUCUUUGAGGGCAUGGUGCACGACUCCAUCAAGGAGGAGCUCCGACCCCUCAUCCAACGAACCUCAUUUGUGAAACGCGCUUUUAAGCGUCUGAAGGAAAACUUUGAGACUGUUGCCCUGUGCUUGACCCUCUUGGCCAACAUAGUGAUCAUGAUCCGUGAAACCCACAAGAGGCAGAAGAUGGUGGAUGGCUCGGUGAAUGAGCACAUCGAGAAGGCAAACAACACCGCAGACGAUGAAACUCUUGACGAGGCGGGAAAGAACCCUCUGGAAACCAGUGGUGCCAGCACCGUUGGCUUCAGAGAGAGAACUCUCACGGACCGAAAGGCGCGUGAUGACGUGGGCUUCGAGCCCGCAUAUCCUGCUGAAGAGUACCCACAAGCUGAAGGACCCUACGCCGGGCCACUCGAGCGUCAGAGACCUCUGAAAGUGAGAGCCAAGCUCCCACAACAGGAGGGACCCUACGCUGGUCCGAUGGAGAGACAGAAACCACUGAAAGUGAAAGUGAAAAACCCGGUCAUUAAGGAAGGACCCUACGAAGGACCGGUGAAGAAGCCUGUCGCUUUGAAAGUGAAAGCCAAGAACUUAAUUGUCACUGAGAGUGGUGCCCCACCGACCGACUUGCAAAAGAUGGUCAUGGGCAACACAAAGCCUGUUGAGCUUAUCCUUGACGGGAAGACAGUAGCCAUCUGCUGUGCUACUGGAGUGUUUGGUACUGCUUACCUCGUGCCUCGUCACCUUUUUGCAGAGAAGUAUGACAAAAUCAUGUUGGACGGCAGAGCCAUGACAGACAGUGACUACAGAGUGUUUGAGUUUGAGAUUAAAGUAAAAGGACAGGACAUGCUCUCGGACGCCGCACUCAUGGUACUCCACCGCGGGAACCGUGUGAGAGACAUCACGAAACACUUUCGUGAUACAGCAAGAAUGAAGAAAGGCACUCCCGUUGUUGGUGUGAUCAACAACGCAGACGUCGGGAGGCUGAUUUUCUCUGGUGAGGCCCUCACCUACAAGGAUAUCGUAGUGUGCAUGGAUGGAGAUACCAUGCCUGGCCUUUUUGCCUAUAAGGCCGCCACCAAGGCCGGUUACUGUGGAGGAGCCGUUCUCGCUAAGGACGGGGCUGACACGUUCAUCGUCGGCACUCACUCUGCAGGAGGCAAUGGAGUAGGAUACUGUUCGUGUGUUUCCAGGUCCAUGCUUCAAAAGAUGAAGGCACACAUUGACCCCGAACCACACCACGAGGGUUUGGUUGUCAACACCAGAGAUGUGGAAGAGCGCGUUCACGUGAUGCGCAAAACCAAGCUUGCACCCACCGUUGCACACGGUGUGUUCAACCCUGACUUUGGGCCUGCCGCCUUGUCUAACAAGGACCCGCGCCUGAACGAGGGUGUUGUCCUCGAUGAAGCCAUCUUCUCCAAACACAAGGGAGACACAAAGAUGUCCGAGGAAGACAAAGCGCUGUUCCGCCGUUGUGCUGCUGACUACGCCUCACGCUUGCACAGCGUACUGGGUACAGCAAAUGCCCCAUUGAGCAUCUACGAGGCAAUCAAGGGCGUUGAUGGACUCGACGCCAUGGAGCCAGACACUGCGCCUGGCCUCCCCUGGGCACUCCAGGGGAAACGUCGUGGCGCGCUCAUCGAUUUUGAGAACGGCACGGUCGGGCCCGAAGUCGAGGCUGCCUUGAAGCUCAUGGAGAGAAGAGAAUACAAAUUUGUUUGCCAGACCUUCCUGAAGGACGAAAUUCGCCCGAUGGAGAAAGUACGUGCCGGCAAGACUCGCAUUGUCGACGUUUUGCCCGUUGAACACAUUCUUUACACCAGGAUGAUGAUUGGCAGAUUCUGUGCACAAAUGCACUCAAACAACGGACCGCAAAUUGGCUCAGCAGUCGGUUGUAACCCUGAUGUUGAUUGGCAAAGAUUUGGCACACACUUCGCCCAAUACAGAAACGUGUGGGACGUGGACUACUCGGCCUUCGAUGCUAACCACUGCAGUGACGCAAUGAACAUCAUGUUUGAGGAAGUGUUCCGCACGGACUUUGGUUUUCACCCAAAUGCUGAGUGGAUCCUGAAGACUCUCGUGAACACGGAACACGCCUAUGAAAAUAAACGUAUCACUGUCGAGGGCGGGAUGCCAUCUGGUUGUUCCGCAACAAGCAUCAUCAACACAAUCUUGAACAACAUAUAUGUGCUCUACGCCUUGCGUAGACACUAUGAGGGAGUUGAGCUGGAUACUUACACCAUGAUCUCAUACGGAGACGACAUCGUGGUGGCAAGUGACUACGAUUUGGACUUUGAGGCUCUCAAGCCCCACUUUAAAUCUCUCGGUCAAACCAUUACUCCAGCUGACAAAAGCGACAAAGGUUUUGUUCUCGGACAUUCCAUCACCGAUGUCACUUUCCUCAAAAGACACUUCCACAUGGAUUAUGGAACUGGGUUUUACAAACCUGUGAUGGCCUCAAAGACUCUCGAGGCCAUUCUCUCCUUUGCACGCCGUGGGACCAUACAGGAGAAGUUGAUCUCCGUGGCAGGACUCGCCGUCCACUCCGGACCAGACGAGUACCGGCGUCUCUUUGAACCCUUCCAGGGCCUCUUUGAGAUUCCAAGCUACAGAUCACUUUACCUGCGUUGGGUGAACGCCGUGUGCGGUGACGCAUAAUCCCUCAGAGAUCACAAUUGGCACAAUGACUCUGGGGCGCGCGACGCCGUAGGAGUGAAAAGCCCGAAGGGCUUCUCCCGCUUCCUUAUCCCAAAAAAAAAAAAAAAA
Lig328 RNA CCCCUCUCCCUCCCCCCCCCCUAACGUUACUGGCCGAAGCCGCUUGGAAUAAGGCCGGUGUGCGUUUGUCUAUAUGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGAAACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGC