P38996_RRM1

Protein information

  • Protein ID: P38996
  • Protein Name: Nuclear polyadenylated RNA-binding protein 3
  • Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
  • Gene Name: NAB3

Domain information

  • Pfam ID: PF00076
  • Pfam Clan Name: RRM
  • Domain Range: 332 - 395
  • Domain Length: 64

Domain Sequence

  • LFIGNLPLKNVSKEDLFRIFSPYGHIMQINIKNAFGFIQFDNPQSVRDAIECESQEMNFGKKLI

Available Structures for this domain - 4 

Experiment ID RRM Structure Experiment Type Ligand ID Contacts
Exp_2KVI 2KVIA01 NMR No contacts
Exp_2L41 2L41A01 NMR Lig40 Contacts
Exp_2XNQ 2XNQA01 X-ray Diffraction No contacts
Exp_2XNR 2XNRA01 X-ray Diffraction Lig36 Contacts

Binding Experiments for this domain - 8 

Experiment ID RRM Entryname Experiment Type Ligand ID Binds? Binding Affinity
Exp_334 P38996_RRM1 Fluorescence polarization Lig36 Yes 110 ± 20 µM
Exp_335 P38996_RRM1 Fluorescence polarization Lig36 Yes 70 ± 20 µM
Exp_336 P38996_RRM1 Fluorescence polarization Lig293 Yes 100 ± 20 µM
Exp_337 P38996_RRM1 Fluorescence polarization Lig293 Yes 70 ± 20 µM
Exp_338 P38996_RRM1 Fluorescence polarization Lig326 Yes 350 ± (+140/-90) µM
Exp_339 P38996_RRM1 Fluorescence polarization Lig326 Yes 470 ± (+130/-90) µM
Exp_340 P38996_RRM1 Fluorescence polarization Lig238 Yes 250 ± (+60/-40) µM
Exp_341 P38996_RRM1 Fluorescence polarization Lig238 Yes 230 ± (+40/-30) µM

Ligands tested for Binding - 5 

Ligand ID Ligand Type Ligand Sequence
Lig238 RNA FAM-UCCCUAUCCCUA
Lig293 RNA FAM-UUCUUAUUUUUA
Lig326 RNA FAM-UUUUUAUUUUUA
Lig36 RNA UUCUUAUUCUUA
Lig40 RNA UCUU