Exp_263

Experiment information

  • Experiment ID: Exp_263
  • PubMed ID : 10856256
  • Experiment Type : Filter binding assay
  • Sequence used : 324-531

Protein involved in this Experiment

UniProt ID Protein Length Mutated Instance
P26599 557 P26599_Mut2

RRM domains involved in this Experiment

RRM Entryname Sequence Range UniProt ID
P26599_RRM3 309 - 433 P26599
P26599_RRM4 456 - 521 P26599

Binding Experiments for this domain - 2 

Experiment ID RRM Entryname Experiment Type Ligand ID Binds? Binding Affinity
Exp_263 P26599_RRM3 Filter binding assay Lig301 Yes 0.15 ± μM
Exp_263 P26599_RRM4 Filter binding assay Lig301 Yes 0.15 ± μM

Ligands tested for Binding - 1 

Ligand ID Ligand Type Ligand Sequence
Lig301 RNA UUGAAAGGGGGCGCUAGGGUUUCACCCCUAGCACGCCAACGACAGUCCCUGCGUUGCACUCCACACUUACGUUCGCGUGCACGCGGGACCCGAUGGACUUUCGUUCACCCACCUACGGUUGGACUCACGGCACCGCGUGGCCAUUUUAGCUGGACCGUGUGGACGAACACCGCUUGCGCUUCUCGCGUGACCGGCUAGUACUCUGACCACUCUCCGCCUACUUGGUCGCUGCGCUGUCUUGGGCACUCCUAUUGGGGUCGUUCGACGCUCCACGGGCUCCCCCGUGCGGCGGGCUACGGUGAUGGGGCCGUUUCGCGCGGGCUGAUCGCCUGGUCUGCUUCGGCUGUCACCCGAAGCCCGCCUUUCACCCCCCCCCCCCUAAGUUUUACCGUCGUUUCCGACGUAAAGGGGAAGUAACCACAAGCUUGACACCGCCUGUCCCGGCGUUAAAGGGAAGUAACCACAAGCUUACAACCGCCUACCCCGGUGUUAAUGGGAUGUAACCACAAGAUACACCUUCACCCGGAAGUAAAACGGCAAAUUCACACAGUUUUGCCCGUUUUUCAUGAGAAACGGGACGUCUGCGCACGAAACGCGCCGUCGCUUGAGGAGGACUUGUACAAACACGAUCUAAACAGGUUUCCCCAACUGACAUACACCGUGCAAUUUGAAACUCCGCCUGGUCUUUCCAGGUCUAGAGGGGUAACACUUUGUACUGUGCUUGACUCCACGCUCGGUCCACUGGCGAGUGUUAGUAACAGCACUGGUGCUUCGUAGCGGAGCAUGGUGGCCGUGGGAACUCCUCCUUGGUAACAAGGACCCACGGGGCCGAAAGCCACGUCCUGACGGACCCACCAUGUGUGCAACCCCAGCACGGCAACUUUUCUGUGAAACUCACUCUAAGGUGACACUGAUACUGGUAUUCAAGUACUGGUGACAGGCUAAGGAUGCCCUUCAGGUACCCCGAGGUAACACGCGACACUCGGGAUCUGAGAAGGGGACUGGGGCUUCUGUAAAAGCGCCCAGUUUAAAAAGCUUCUAUGCCUGGAUAGGUGACCGGAGGCCGGCGCCUUUCCAUUAUAACUACUGACUUUAUGAACACAACUAACUGUUUUAUCGCUUUGGUGUACGCUAUCAGAGAGAUCAGAACGCUUUUCCUUUCACGAGCUACAGGAAAGAUGGAAUUCACGCUGUACAACGGUGAAAAGAAGACCUUUUACUCCAGGCCCAACAACCACGACAACUGUUGGUUGAACACCAUCCUUCAGUUGUUCAGGUACGUCGAUGAGCCUUUCUUCGACUGGGUCUACAACUCACCCCAGAACCUCACGCUUGAAGCCAUCAAACAGCUGGAAGAACUCACAGGGCUUGAGUUACACGAAGGCGGACCGCCUGCCGCUGUUGUUUGGAACAUCAAACACUUGCUUCAAACCGGUAUUGGUACCGCCUCGCGACCUAGCGAGGUGUGUGUGGUGGACGGUACGAACAUGUGUCUGGCUGAUUUCCAUGCAGGCAUCUUCCUGAAAGGACAGGAACAUGCCGUGUUUGCUUGUGUCACCUCCAACGGGUGGUACGCGAUUGACGAUGAGGACUUCUACCCAUGGACGCCGGACCCGUCCGACGUUCUGGUGUUCGUUCCAUAUGAUCAAGAACCACUCAACGGAGAAUGGAAAAUCAACGUUCAGCGGAAGCUCAAGGGAGCUGGACAAUCCAGUCCGGCGACCGGCUCGCAGAACCAAUCUGGCAAUACUGGCAGCAUAAUCAACAACUACUACAUGCAACAGUACCAGAACUCCAUGGACACACAACUUGGUGACAACGCCAUCAGCGGAGGCUCCAACGAGGGCUCCACAGACACAACUUCCAAUCACACAACCAACACUCAGAACAACGACUGGUUUUCCAAGCUCGCAGGUUCUGCCUUCAGCGGUCUUUUUGGCGCCCUUCUUGCUGACAAAAAGACAGAGGAAACCACUCUCCUCGAGGACCGCAUCCUCACUACCCGCAACGGACACACGACCUCAACAACACAGUCGAGUGUCGGAGUCACGUACGGGUAUGCAACAGCUGAGGACUUUGUGAGUGGGCCAAACACUUCUGGUCUUGAAACCAGAGUUGUCCAGGCAGAACGCUUCUUCAAAACCCACCUCUUCGAUUGGGUCACCAGUGACCCAUUCGGACGCUGCCACCUGUUGGAACUCCCAACUGACCACAAAGGUGUCUACGGAAGUCUGACCGACUCGUACGCAUAUAUGAGAAACGGUUGGGACGUUGAAGUCACCGCGGUUGGCAACCAGUUCAACGGAGGGUGUCUGUUGGUGGCGAUGGUGCCAGAGCUUUGUUCCAUCCAGAAGAGGGAGCUGUACCAGCUCACGCUCUUCCCCCAUCAGUUCAUCAACCCACGCACAAACAUGACUGCGCACAUCACUGUGCCUUUCGUUGGCGUCAAUCGCUACGACCAGUACAAGGUGCACAAACCUUGGACCCUCGUGGUUAUGGUCGUAGCCCCCCUGACUGUCAACACUGAAGGCGCUCCACAGAUCAAGGUGUACGCCAACAUUGCCCCAACCAACGUGUACGUAGCGGGCGAGUUUCCUUCCAAAGAGGGAAUUUUCCCCGUAGCUUGCAGUGACGGCUAUGGUGGCCUGGUGACCACGGACCCGAAGACGGCUGACCCCGUUUACGGGAAAGUGUUUAACCCCCCACGCAACCUGCUGCCUGGGCGUUUUACCAAUCUCCUUGAUGUGGCUGAGGCAUGCCCGACGUUUCUGCACUUCGAGGGUGACGUGCCAUACGUGACCACGAAAACAGAUUCAGACAGGGUGCUUGCCCAGUUUGACGUGUCCUUAGCAGCAAAGCACAUGUCGAACACCUUUCUUGCAGGUCUCGCGCAGUACUAUGCACAGUACAGUGGCACCAUCAACCUGCACUUCAUGUUUACAGGACCCACUGACGCGAAAGCGCGUUACAUGGUUGCUUACGCCCCACCGGGCAUGGAGCCGCCCCGGACGCCUGAGGCGGCUGCACACUGCAUUCAUGCUGAGUGGGACACUGGGCUAAACUCAAAGUUCACUUUCUCCAUCCCCUAUCUUUCGGCUGCUGACUACGCAUACACCGCGUCAGACGUGGCUGAAACCACGAACGUGCAAGGAUGGGUCUGCUUGUUUCAAAUCACGCACGGCAAAGCCGACGGCGACGCUUUGGUCGUGUUGGCUAGCGCUGGCAAGGACUUUGAGCUGAGGCUGCCGGUCGACGCCCGCAGAGAAACUACCUCUGCGGGUGAAUCAGCAGAUCCCGUGACUACCACUGUUGAGAACUACGGUGGUGAGACACAGGUCCAGAGGCGCCAACACACAGACGUCUCGUUCAUCAUGGACAGAUUCGUGAAAGUGACACCGCAAGAUCAAAUCAACGUUUUGGACCUUAUGCAGAUCCCUGCCCACACGCUGGUGGGAGCGCUCCUGCGCGCGUCCACUUACUACUUCUCUGACCUGGAGAUAGCAGUAAAGCACGAGGGAGACCUCACUUGGGUCCCGAACGGGGCGCCUGAAGCAGCGCUGGAGAACACCACCAACCCAACAGCUUACCACAAGGCACCACUCACUCGGCUCGCCCUGCCUUACACGGCACCACACCGCGUGUUGGCAACCGUGUAUAAUGGAGUGUGCAAGUACAGUAAAACUGCUGUGCCCAACGUGAGAGGUGACACCCAGGUGUUGGAUCAGAAGGCGUCGCGGGCGCUACCCACCUCCUUCAACUACGGUGCCAUCAAAGCAACCCGAGUUAACGAGCUACUUUAUCGGAUGAAGAGGGCUGAGACAUACUGUCCCAGGCCCUUGCUGGCUAUCCAUCCAGCCGAAGCCAGACACAAACAGAAAAUUGUGGCACCUGUGAAACAGACUUUGAACUUUGACCUUCUCAAGUUGGCUGGAGACGUGGAGUCCAACCCUGGGCCCUUCUUCUUCUCCGACGUCAGGUCAAAUUUCUCCAAAUUGGUAGAAACCAUCAACCAGAUGCAGGAGGACAUCUCGACAAAACACGGGCCUGACUUUAACCGGUUGGUGUCUGCGUUUGAGGAAUUGGCUACUGGAGUGAAAGCAAUCAGGACCGGUCUCGACGAGGCCAAACCCUGGUACAAGCUUAUUAAGCUCCUAAGCCGCCUGUCGUGUAUGGCCGCUGUGGCAGCACGGUCCAAGGACCCAGUCCUUGUGGCCAUCAUGCUGGCCGACACCGGUCUCGAGAUUCUGGACAGCACCUUUGUCGUGAAGAAAAUUUCCGAUUCGCUCUCCAGUCUCUUUCACGUGCCGGCCCCCGUCUUCAGUUUCGGAGCCCCGAUCCUGUUGGCCGGGUUGGUCAAGGUCGCCUCGAGUUUCUUCCGGUCCACACCCGAAGACCUUGAGAGAGCAGAGAAACAGCUCAAAGCACGUGACAUUAACGACAUUUUCGCCGUUCUCAAGAACGGCGAGUGGCUGGUCAAGUUGAUCCUUGCCAUCCGCGACUGGAUUAAGGCAUGGAUCGCCUCAGAGGAGAAGUUCGUCACCAUGACAGACCUGGUGCCUGGUAUCCUUGAAAAGCAGCGGGACCUCAACGACCCAAGCAAGUACAAGGAAGCCAAGGAGUGGCUCGACAACGCGCGCCAAGCGUGUUUGAAGAACGGGAACGUCCACAUCGCUAACCUGUGCAAAGUGGUCGCCCCAGCACCCAGCGAGUCGAGACCCGAACCCGUGGUCGUUUGCCUCCGUGGCAAGUCCGGCCAGGGCAAGAGUUUCCUUGCGAACGUGCUCGCGCAAGCAAUCUCCACUCACUUUACUGGUAGAACCAAUUCCGUUUGGUACUGCCCACCUGACCCUGACCACUUCGACGGCUACAACCAGCAGACCGUUGUAGUGAUGGAUGACCUGGGUCAGAACCCCGAUGGCAAGGACUUCAAGUACUUCGCCCAAAUGGUUUCAACCACGGGGUUCAUCCCGCCCAUGGCGUCACUCGAGGACAAAGGCAAACCUUUCAACAGCAAGGUCAUCAUCGCAACCACCAACCUGUACUCGGGUUUUACCCCGAAGACUAUGGUGUGCCCUGAUGCGCUGAACCGGAGGUUCCACUUUGACAUCGACGUGAGCGCCAAGGACGGGUACAAAAUUAACAACAAACUGGACAUCGUCAAAGCACUUGAAGACACUCACACCAACCCGGUAGCAAUGUUUCAGUACGACUGUGCCCUUCUCAACGGCACGGCCGUUGAAAUGAAGAGAAUGCAGCAGGACUUGUUCAAGCCUCAACCACCCCUCCAGAACGUGUACCAGCUCGUUCAGGAGGUGAUUGAACGGGUCGAGCUCCACGAGAAAGUGUCGAGCCACCCGAUUUUCAAGCAGAUCUCAAUUCCUUCCCAAAAAUCCGUGUUGUACUUCCUCAUUGAGAAAGGUCAACACGAGGCGGCAAUUGAAUUCUUUGAGGGCAUGGUGCACGACUCCAUCAAGGAGGAGCUCCGACCCCUCAUCCAACGAACCUCAUUUGUGAAACGCGCUUUUAAGCGUCUGAAGGAAAACUUUGAGACUGUUGCCCUGUGCUUGACCCUCUUGGCCAACAUAGUGAUCAUGAUCCGUGAAACCCACAAGAGGCAGAAGAUGGUGGAUGGCUCGGUGAAUGAGCACAUCGAGAAGGCAAACAACACCGCAGACGAUGAAACUCUUGACGAGGCGGGAAAGAACCCUCUGGAAACCAGUGGUGCCAGCACCGUUGGCUUCAGAGAGAGAACUCUCACGGACCGAAAGGCGCGUGAUGACGUGGGCUUCGAGCCCGCAUAUCCUGCUGAAGAGUACCCACAAGCUGAAGGACCCUACGCCGGGCCACUCGAGCGUCAGAGACCUCUGAAAGUGAGAGCCAAGCUCCCACAACAGGAGGGACCCUACGCUGGUCCGAUGGAGAGACAGAAACCACUGAAAGUGAAAGUGAAAAACCCGGUCAUUAAGGAAGGACCCUACGAAGGACCGGUGAAGAAGCCUGUCGCUUUGAAAGUGAAAGCCAAGAACUUAAUUGUCACUGAGAGUGGUGCCCCACCGACCGACUUGCAAAAGAUGGUCAUGGGCAACACAAAGCCUGUUGAGCUUAUCCUUGACGGGAAGACAGUAGCCAUCUGCUGUGCUACUGGAGUGUUUGGUACUGCUUACCUCGUGCCUCGUCACCUUUUUGCAGAGAAGUAUGACAAAAUCAUGUUGGACGGCAGAGCCAUGACAGACAGUGACUACAGAGUGUUUGAGUUUGAGAUUAAAGUAAAAGGACAGGACAUGCUCUCGGACGCCGCACUCAUGGUACUCCACCGCGGGAACCGUGUGAGAGACAUCACGAAACACUUUCGUGAUACAGCAAGAAUGAAGAAAGGCACUCCCGUUGUUGGUGUGAUCAACAACGCAGACGUCGGGAGGCUGAUUUUCUCUGGUGAGGCCCUCACCUACAAGGAUAUCGUAGUGUGCAUGGAUGGAGAUACCAUGCCUGGCCUUUUUGCCUAUAAGGCCGCCACCAAGGCCGGUUACUGUGGAGGAGCCGUUCUCGCUAAGGACGGGGCUGACACGUUCAUCGUCGGCACUCACUCUGCAGGAGGCAAUGGAGUAGGAUACUGUUCGUGUGUUUCCAGGUCCAUGCUUCAAAAGAUGAAGGCACACAUUGACCCCGAACCACACCACGAGGGUUUGGUUGUCAACACCAGAGAUGUGGAAGAGCGCGUUCACGUGAUGCGCAAAACCAAGCUUGCACCCACCGUUGCACACGGUGUGUUCAACCCUGACUUUGGGCCUGCCGCCUUGUCUAACAAGGACCCGCGCCUGAACGAGGGUGUUGUCCUCGAUGAAGCCAUCUUCUCCAAACACAAGGGAGACACAAAGAUGUCCGAGGAAGACAAAGCGCUGUUCCGCCGUUGUGCUGCUGACUACGCCUCACGCUUGCACAGCGUACUGGGUACAGCAAAUGCCCCAUUGAGCAUCUACGAGGCAAUCAAGGGCGUUGAUGGACUCGACGCCAUGGAGCCAGACACUGCGCCUGGCCUCCCCUGGGCACUCCAGGGGAAACGUCGUGGCGCGCUCAUCGAUUUUGAGAACGGCACGGUCGGGCCCGAAGUCGAGGCUGCCUUGAAGCUCAUGGAGAGAAGAGAAUACAAAUUUGUUUGCCAGACCUUCCUGAAGGACGAAAUUCGCCCGAUGGAGAAAGUACGUGCCGGCAAGACUCGCAUUGUCGACGUUUUGCCCGUUGAACACAUUCUUUACACCAGGAUGAUGAUUGGCAGAUUCUGUGCACAAAUGCACUCAAACAACGGACCGCAAAUUGGCUCAGCAGUCGGUUGUAACCCUGAUGUUGAUUGGCAAAGAUUUGGCACACACUUCGCCCAAUACAGAAACGUGUGGGACGUGGACUACUCGGCCUUCGAUGCUAACCACUGCAGUGACGCAAUGAACAUCAUGUUUGAGGAAGUGUUCCGCACGGACUUUGGUUUUCACCCAAAUGCUGAGUGGAUCCUGAAGACUCUCGUGAACACGGAACACGCCUAUGAAAAUAAACGUAUCACUGUCGAGGGCGGGAUGCCAUCUGGUUGUUCCGCAACAAGCAUCAUCAACACAAUCUUGAACAACAUAUAUGUGCUCUACGCCUUGCGUAGACACUAUGAGGGAGUUGAGCUGGAUACUUACACCAUGAUCUCAUACGGAGACGACAUCGUGGUGGCAAGUGACUACGAUUUGGACUUUGAGGCUCUCAAGCCCCACUUUAAAUCUCUCGGUCAAACCAUUACUCCAGCUGACAAAAGCGACAAAGGUUUUGUUCUCGGACAUUCCAUCACCGAUGUCACUUUCCUCAAAAGACACUUCCACAUGGAUUAUGGAACUGGGUUUUACAAACCUGUGAUGGCCUCAAAGACUCUCGAGGCCAUUCUCUCCUUUGCACGCCGUGGGACCAUACAGGAGAAGUUGAUCUCCGUGGCAGGACUCGCCGUCCACUCCGGACCAGACGAGUACCGGCGUCUCUUUGAACCCUUCCAGGGCCUCUUUGAGAUUCCAAGCUACAGAUCACUUUACCUGCGUUGGGUGAACGCCGUGUGCGGUGACGCAUAAUCCCUCAGAGAUCACAAUUGGCACAAUGACUCUGGGGCGCGCGACGCCGUAGGAGUGAAAAGCCCGAAGGGCUUCUCCCGCUUCCUUAUCCCAAAAAAAAAAAAAAAA