P26599_RRM3

Protein information

Protein ID: P26599
Protein Name: Polypyrimidine tract-binding protein 1
Organism: Homo sapiens
Gene Name: PTBP1

Domain information

Pfam ID: PF13893
Pfam Clan Name: RRM
Domain Range: 309 - 433
Domain Length: 125

Domain Sequence

AGAGFPPTFAIPQAAGLSVPNVHGALAPLAIPSAAAAAAAAGRIAIPGLAGAGNSVLLVSNLNPERVTPQSLFILFGVYGDVQRVKILFNKKENALVQMADGNQAQLAMSHLNGHKLHGKPIRIT

Available Structures for this domain - 3

Experiment ID	RRM Structure	Experiment Type	Ligand ID	Contacts
Exp_1QM9	1QM9A01	NMR		No contacts
Exp_2ADC	2ADCA01	NMR	Lig223	Contacts
Exp_2EVZ	2EVZA01	NMR		No contacts

Binding Experiments for this domain - 38

Experiment ID	RRM Entryname	Experiment Type	Ligand ID	Binds?	Binding Affinity
Exp_253	P26599_RRM3	Filter binding assay	Lig328	Yes	0.026 ± μM
Exp_254	P26599_RRM3	Filter binding assay	Lig328	Yes	0.18 ± μM
Exp_255	P26599_RRM3	Filter binding assay	Lig328	Yes	0.23 ± μM
Exp_256	P26599_RRM3	Filter binding assay	Lig328	Yes	0.82 ± μM
Exp_257	P26599_RRM3	Filter binding assay	Lig328	Yes	0.62 ± μM
Exp_259	P26599_RRM3	Filter binding assay	Lig232	Yes	0.038 ± μM
Exp_260	P26599_RRM3	Filter binding assay	Lig232	Yes	0.3 ± μM
Exp_261	P26599_RRM3	Filter binding assay	Lig232	Yes	0.21 ± μM
Exp_262	P26599_RRM3	Filter binding assay	Lig301	Yes	0.061 ± μM
Exp_263	P26599_RRM3	Filter binding assay	Lig301	Yes	0.15 ± μM
Exp_264	P26599_RRM3	Filter binding assay	Lig301	Yes	0.15 ± μM
Exp_265	P26599_RRM3	Filter binding assay	Lig328	Yes	0.21 ± μM
Exp_266	P26599_RRM3	Filter binding assay	Lig328	Yes	0.28 ± μM
Exp_267	P26599_RRM3	Filter binding assay	Lig328	Yes	0.24 ± μM
Exp_268	P26599_RRM3	Filter binding assay	Lig328	Yes	0.56 ± μM
Exp_269	P26599_RRM3	Filter binding assay	Lig328	Yes	0.46 ± μM
Exp_270	P26599_RRM3	Filter binding assay	Lig328	Yes	0.7 ± μM
Exp_271	P26599_RRM3	Filter binding assay	Lig328	Yes	0.17 ± μM
Exp_272	P26599_RRM3	Filter binding assay	Lig328	Yes	0.55 ± μM
Exp_273	P26599_RRM3	Filter binding assay	Lig328	Yes	0.74 ± μM
Exp_274	P26599_RRM3	Filter binding assay	Lig232	Yes	0.23 ± μM
Exp_275	P26599_RRM3	Filter binding assay	Lig232	Yes	0.39 ± μM
Exp_276	P26599_RRM3	Filter binding assay	Lig232	Yes	0.19 ± μM
Exp_277	P26599_RRM3	Filter binding assay	Lig232	Yes	1.01 ± μM
Exp_278	P26599_RRM3	Filter binding assay	Lig232	Yes	0.55 ± μM
Exp_279	P26599_RRM3	Filter binding assay	Lig232	Yes	0.78 ± μM
Exp_280	P26599_RRM3	Filter binding assay	Lig232	Yes	0.19 ± μM
Exp_281	P26599_RRM3	Filter binding assay	Lig232	Yes	0.73 ± μM
Exp_282	P26599_RRM3	Filter binding assay	Lig232	Yes	0.59 ± μM
Exp_283	P26599_RRM3	Filter binding assay	Lig328	Yes	0.52 ± μM
Exp_284	P26599_RRM3	Filter binding assay	Lig328	Yes	0.18 ± μM
Exp_285	P26599_RRM3	Filter binding assay	Lig328	Yes	0.37 ± μM
Exp_286	P26599_RRM3	Filter binding assay	Lig328	Yes	0.35 ± μM
Exp_287	P26599_RRM3	Filter binding assay	Lig328	Yes	0.16 ± μM
Exp_288	P26599_RRM3	Filter binding assay	Lig232	Yes	0.46 ± μM
Exp_289	P26599_RRM3	Filter binding assay	Lig232	Yes	0.16 ± μM
Exp_290	P26599_RRM3	Filter binding assay	Lig232	Yes	0.67 ± μM
Exp_291	P26599_RRM3	Filter binding assay	Lig232	Yes	0.19 ± μM

Ligands tested for Binding - 4

Ligand ID	Ligand Type	Ligand Sequence
Lig301	RNA	UUGAAAGGGGGCGCUAGGGUUUCACCCCUAGCACGCCAACGACAGUCCCUGCGUUGCACUCCACACUUACGUUCGCGUGCACGCGGGACCCGAUGGACUUUCGUUCACCCACCUACGGUUGGACUCACGGCACCGCGUGGCCAUUUUAGCUGGACCGUGUGGACGAACACCGCUUGCGCUUCUCGCGUGACCGGCUAGUACUCUGACCACUCUCCGCCUACUUGGUCGCUGCGCUGUCUUGGGCACUCCUAUUGGGGUCGUUCGACGCUCCACGGGCUCCCCCGUGCGGCGGGCUACGGUGAUGGGGCCGUUUCGCGCGGGCUGAUCGCCUGGUCUGCUUCGGCUGUCACCCGAAGCCCGCCUUUCACCCCCCCCCCCCUAAGUUUUACCGUCGUUUCCGACGUAAAGGGGAAGUAACCACAAGCUUGACACCGCCUGUCCCGGCGUUAAAGGGAAGUAACCACAAGCUUACAACCGCCUACCCCGGUGUUAAUGGGAUGUAACCACAAGAUACACCUUCACCCGGAAGUAAAACGGCAAAUUCACACAGUUUUGCCCGUUUUUCAUGAGAAACGGGACGUCUGCGCACGAAACGCGCCGUCGCUUGAGGAGGACUUGUACAAACACGAUCUAAACAGGUUUCCCCAACUGACAUACACCGUGCAAUUUGAAACUCCGCCUGGUCUUUCCAGGUCUAGAGGGGUAACACUUUGUACUGUGCUUGACUCCACGCUCGGUCCACUGGCGAGUGUUAGUAACAGCACUGGUGCUUCGUAGCGGAGCAUGGUGGCCGUGGGAACUCCUCCUUGGUAACAAGGACCCACGGGGCCGAAAGCCACGUCCUGACGGACCCACCAUGUGUGCAACCCCAGCACGGCAACUUUUCUGUGAAACUCACUCUAAGGUGACACUGAUACUGGUAUUCAAGUACUGGUGACAGGCUAAGGAUGCCCUUCAGGUACCCCGAGGUAACACGCGACACUCGGGAUCUGAGAAGGGGACUGGGGCUUCUGUAAAAGCGCCCAGUUUAAAAAGCUUCUAUGCCUGGAUAGGUGACCGGAGGCCGGCGCCUUUCCAUUAUAACUACUGACUUUAUGAACACAACUAACUGUUUUAUCGCUUUGGUGUACGCUAUCAGAGAGAUCAGAACGCUUUUCCUUUCACGAGCUACAGGAAAGAUGGAAUUCACGCUGUACAACGGUGAAAAGAAGACCUUUUACUCCAGGCCCAACAACCACGACAACUGUUGGUUGAACACCAUCCUUCAGUUGUUCAGGUACGUCGAUGAGCCUUUCUUCGACUGGGUCUACAACUCACCCCAGAACCUCACGCUUGAAGCCAUCAAACAGCUGGAAGAACUCACAGGGCUUGAGUUACACGAAGGCGGACCGCCUGCCGCUGUUGUUUGGAACAUCAAACACUUGCUUCAAACCGGUAUUGGUACCGCCUCGCGACCUAGCGAGGUGUGUGUGGUGGACGGUACGAACAUGUGUCUGGCUGAUUUCCAUGCAGGCAUCUUCCUGAAAGGACAGGAACAUGCCGUGUUUGCUUGUGUCACCUCCAACGGGUGGUACGCGAUUGACGAUGAGGACUUCUACCCAUGGACGCCGGACCCGUCCGACGUUCUGGUGUUCGUUCCAUAUGAUCAAGAACCACUCAACGGAGAAUGGAAAAUCAACGUUCAGCGGAAGCUCAAGGGAGCUGGACAAUCCAGUCCGGCGACCGGCUCGCAGAACCAAUCUGGCAAUACUGGCAGCAUAAUCAACAACUACUACAUGCAACAGUACCAGAACUCCAUGGACACACAACUUGGUGACAACGCCAUCAGCGGAGGCUCCAACGAGGGCUCCACAGACACAACUUCCAAUCACACAACCAACACUCAGAACAACGACUGGUUUUCCAAGCUCGCAGGUUCUGCCUUCAGCGGUCUUUUUGGCGCCCUUCUUGCUGACAAAAAGACAGAGGAAACCACUCUCCUCGAGGACCGCAUCCUCACUACCCGCAACGGACACACGACCUCAACAACACAGUCGAGUGUCGGAGUCACGUACGGGUAUGCAACAGCUGAGGACUUUGUGAGUGGGCCAAACACUUCUGGUCUUGAAACCAGAGUUGUCCAGGCAGAACGCUUCUUCAAAACCCACCUCUUCGAUUGGGUCACCAGUGACCCAUUCGGACGCUGCCACCUGUUGGAACUCCCAACUGACCACAAAGGUGUCUACGGAAGUCUGACCGACUCGUACGCAUAUAUGAGAAACGGUUGGGACGUUGAAGUCACCGCGGUUGGCAACCAGUUCAACGGAGGGUGUCUGUUGGUGGCGAUGGUGCCAGAGCUUUGUUCCAUCCAGAAGAGGGAGCUGUACCAGCUCACGCUCUUCCCCCAUCAGUUCAUCAACCCACGCACAAACAUGACUGCGCACAUCACUGUGCCUUUCGUUGGCGUCAAUCGCUACGACCAGUACAAGGUGCACAAACCUUGGACCCUCGUGGUUAUGGUCGUAGCCCCCCUGACUGUCAACACUGAAGGCGCUCCACAGAUCAAGGUGUACGCCAACAUUGCCCCAACCAACGUGUACGUAGCGGGCGAGUUUCCUUCCAAAGAGGGAAUUUUCCCCGUAGCUUGCAGUGACGGCUAUGGUGGCCUGGUGACCACGGACCCGAAGACGGCUGACCCCGUUUACGGGAAAGUGUUUAACCCCCCACGCAACCUGCUGCCUGGGCGUUUUACCAAUCUCCUUGAUGUGGCUGAGGCAUGCCCGACGUUUCUGCACUUCGAGGGUGACGUGCCAUACGUGACCACGAAAACAGAUUCAGACAGGGUGCUUGCCCAGUUUGACGUGUCCUUAGCAGCAAAGCACAUGUCGAACACCUUUCUUGCAGGUCUCGCGCAGUACUAUGCACAGUACAGUGGCACCAUCAACCUGCACUUCAUGUUUACAGGACCCACUGACGCGAAAGCGCGUUACAUGGUUGCUUACGCCCCACCGGGCAUGGAGCCGCCCCGGACGCCUGAGGCGGCUGCACACUGCAUUCAUGCUGAGUGGGACACUGGGCUAAACUCAAAGUUCACUUUCUCCAUCCCCUAUCUUUCGGCUGCUGACUACGCAUACACCGCGUCAGACGUGGCUGAAACCACGAACGUGCAAGGAUGGGUCUGCUUGUUUCAAAUCACGCACGGCAAAGCCGACGGCGACGCUUUGGUCGUGUUGGCUAGCGCUGGCAAGGACUUUGAGCUGAGGCUGCCGGUCGACGCCCGCAGAGAAACUACCUCUGCGGGUGAAUCAGCAGAUCCCGUGACUACCACUGUUGAGAACUACGGUGGUGAGACACAGGUCCAGAGGCGCCAACACACAGACGUCUCGUUCAUCAUGGACAGAUUCGUGAAAGUGACACCGCAAGAUCAAAUCAACGUUUUGGACCUUAUGCAGAUCCCUGCCCACACGCUGGUGGGAGCGCUCCUGCGCGCGUCCACUUACUACUUCUCUGACCUGGAGAUAGCAGUAAAGCACGAGGGAGACCUCACUUGGGUCCCGAACGGGGCGCCUGAAGCAGCGCUGGAGAACACCACCAACCCAACAGCUUACCACAAGGCACCACUCACUCGGCUCGCCCUGCCUUACACGGCACCACACCGCGUGUUGGCAACCGUGUAUAAUGGAGUGUGCAAGUACAGUAAAACUGCUGUGCCCAACGUGAGAGGUGACACCCAGGUGUUGGAUCAGAAGGCGUCGCGGGCGCUACCCACCUCCUUCAACUACGGUGCCAUCAAAGCAACCCGAGUUAACGAGCUACUUUAUCGGAUGAAGAGGGCUGAGACAUACUGUCCCAGGCCCUUGCUGGCUAUCCAUCCAGCCGAAGCCAGACACAAACAGAAAAUUGUGGCACCUGUGAAACAGACUUUGAACUUUGACCUUCUCAAGUUGGCUGGAGACGUGGAGUCCAACCCUGGGCCCUUCUUCUUCUCCGACGUCAGGUCAAAUUUCUCCAAAUUGGUAGAAACCAUCAACCAGAUGCAGGAGGACAUCUCGACAAAACACGGGCCUGACUUUAACCGGUUGGUGUCUGCGUUUGAGGAAUUGGCUACUGGAGUGAAAGCAAUCAGGACCGGUCUCGACGAGGCCAAACCCUGGUACAAGCUUAUUAAGCUCCUAAGCCGCCUGUCGUGUAUGGCCGCUGUGGCAGCACGGUCCAAGGACCCAGUCCUUGUGGCCAUCAUGCUGGCCGACACCGGUCUCGAGAUUCUGGACAGCACCUUUGUCGUGAAGAAAAUUUCCGAUUCGCUCUCCAGUCUCUUUCACGUGCCGGCCCCCGUCUUCAGUUUCGGAGCCCCGAUCCUGUUGGCCGGGUUGGUCAAGGUCGCCUCGAGUUUCUUCCGGUCCACACCCGAAGACCUUGAGAGAGCAGAGAAACAGCUCAAAGCACGUGACAUUAACGACAUUUUCGCCGUUCUCAAGAACGGCGAGUGGCUGGUCAAGUUGAUCCUUGCCAUCCGCGACUGGAUUAAGGCAUGGAUCGCCUCAGAGGAGAAGUUCGUCACCAUGACAGACCUGGUGCCUGGUAUCCUUGAAAAGCAGCGGGACCUCAACGACCCAAGCAAGUACAAGGAAGCCAAGGAGUGGCUCGACAACGCGCGCCAAGCGUGUUUGAAGAACGGGAACGUCCACAUCGCUAACCUGUGCAAAGUGGUCGCCCCAGCACCCAGCGAGUCGAGACCCGAACCCGUGGUCGUUUGCCUCCGUGGCAAGUCCGGCCAGGGCAAGAGUUUCCUUGCGAACGUGCUCGCGCAAGCAAUCUCCACUCACUUUACUGGUAGAACCAAUUCCGUUUGGUACUGCCCACCUGACCCUGACCACUUCGACGGCUACAACCAGCAGACCGUUGUAGUGAUGGAUGACCUGGGUCAGAACCCCGAUGGCAAGGACUUCAAGUACUUCGCCCAAAUGGUUUCAACCACGGGGUUCAUCCCGCCCAUGGCGUCACUCGAGGACAAAGGCAAACCUUUCAACAGCAAGGUCAUCAUCGCAACCACCAACCUGUACUCGGGUUUUACCCCGAAGACUAUGGUGUGCCCUGAUGCGCUGAACCGGAGGUUCCACUUUGACAUCGACGUGAGCGCCAAGGACGGGUACAAAAUUAACAACAAACUGGACAUCGUCAAAGCACUUGAAGACACUCACACCAACCCGGUAGCAAUGUUUCAGUACGACUGUGCCCUUCUCAACGGCACGGCCGUUGAAAUGAAGAGAAUGCAGCAGGACUUGUUCAAGCCUCAACCACCCCUCCAGAACGUGUACCAGCUCGUUCAGGAGGUGAUUGAACGGGUCGAGCUCCACGAGAAAGUGUCGAGCCACCCGAUUUUCAAGCAGAUCUCAAUUCCUUCCCAAAAAUCCGUGUUGUACUUCCUCAUUGAGAAAGGUCAACACGAGGCGGCAAUUGAAUUCUUUGAGGGCAUGGUGCACGACUCCAUCAAGGAGGAGCUCCGACCCCUCAUCCAACGAACCUCAUUUGUGAAACGCGCUUUUAAGCGUCUGAAGGAAAACUUUGAGACUGUUGCCCUGUGCUUGACCCUCUUGGCCAACAUAGUGAUCAUGAUCCGUGAAACCCACAAGAGGCAGAAGAUGGUGGAUGGCUCGGUGAAUGAGCACAUCGAGAAGGCAAACAACACCGCAGACGAUGAAACUCUUGACGAGGCGGGAAAGAACCCUCUGGAAACCAGUGGUGCCAGCACCGUUGGCUUCAGAGAGAGAACUCUCACGGACCGAAAGGCGCGUGAUGACGUGGGCUUCGAGCCCGCAUAUCCUGCUGAAGAGUACCCACAAGCUGAAGGACCCUACGCCGGGCCACUCGAGCGUCAGAGACCUCUGAAAGUGAGAGCCAAGCUCCCACAACAGGAGGGACCCUACGCUGGUCCGAUGGAGAGACAGAAACCACUGAAAGUGAAAGUGAAAAACCCGGUCAUUAAGGAAGGACCCUACGAAGGACCGGUGAAGAAGCCUGUCGCUUUGAAAGUGAAAGCCAAGAACUUAAUUGUCACUGAGAGUGGUGCCCCACCGACCGACUUGCAAAAGAUGGUCAUGGGCAACACAAAGCCUGUUGAGCUUAUCCUUGACGGGAAGACAGUAGCCAUCUGCUGUGCUACUGGAGUGUUUGGUACUGCUUACCUCGUGCCUCGUCACCUUUUUGCAGAGAAGUAUGACAAAAUCAUGUUGGACGGCAGAGCCAUGACAGACAGUGACUACAGAGUGUUUGAGUUUGAGAUUAAAGUAAAAGGACAGGACAUGCUCUCGGACGCCGCACUCAUGGUACUCCACCGCGGGAACCGUGUGAGAGACAUCACGAAACACUUUCGUGAUACAGCAAGAAUGAAGAAAGGCACUCCCGUUGUUGGUGUGAUCAACAACGCAGACGUCGGGAGGCUGAUUUUCUCUGGUGAGGCCCUCACCUACAAGGAUAUCGUAGUGUGCAUGGAUGGAGAUACCAUGCCUGGCCUUUUUGCCUAUAAGGCCGCCACCAAGGCCGGUUACUGUGGAGGAGCCGUUCUCGCUAAGGACGGGGCUGACACGUUCAUCGUCGGCACUCACUCUGCAGGAGGCAAUGGAGUAGGAUACUGUUCGUGUGUUUCCAGGUCCAUGCUUCAAAAGAUGAAGGCACACAUUGACCCCGAACCACACCACGAGGGUUUGGUUGUCAACACCAGAGAUGUGGAAGAGCGCGUUCACGUGAUGCGCAAAACCAAGCUUGCACCCACCGUUGCACACGGUGUGUUCAACCCUGACUUUGGGCCUGCCGCCUUGUCUAACAAGGACCCGCGCCUGAACGAGGGUGUUGUCCUCGAUGAAGCCAUCUUCUCCAAACACAAGGGAGACACAAAGAUGUCCGAGGAAGACAAAGCGCUGUUCCGCCGUUGUGCUGCUGACUACGCCUCACGCUUGCACAGCGUACUGGGUACAGCAAAUGCCCCAUUGAGCAUCUACGAGGCAAUCAAGGGCGUUGAUGGACUCGACGCCAUGGAGCCAGACACUGCGCCUGGCCUCCCCUGGGCACUCCAGGGGAAACGUCGUGGCGCGCUCAUCGAUUUUGAGAACGGCACGGUCGGGCCCGAAGUCGAGGCUGCCUUGAAGCUCAUGGAGAGAAGAGAAUACAAAUUUGUUUGCCAGACCUUCCUGAAGGACGAAAUUCGCCCGAUGGAGAAAGUACGUGCCGGCAAGACUCGCAUUGUCGACGUUUUGCCCGUUGAACACAUUCUUUACACCAGGAUGAUGAUUGGCAGAUUCUGUGCACAAAUGCACUCAAACAACGGACCGCAAAUUGGCUCAGCAGUCGGUUGUAACCCUGAUGUUGAUUGGCAAAGAUUUGGCACACACUUCGCCCAAUACAGAAACGUGUGGGACGUGGACUACUCGGCCUUCGAUGCUAACCACUGCAGUGACGCAAUGAACAUCAUGUUUGAGGAAGUGUUCCGCACGGACUUUGGUUUUCACCCAAAUGCUGAGUGGAUCCUGAAGACUCUCGUGAACACGGAACACGCCUAUGAAAAUAAACGUAUCACUGUCGAGGGCGGGAUGCCAUCUGGUUGUUCCGCAACAAGCAUCAUCAACACAAUCUUGAACAACAUAUAUGUGCUCUACGCCUUGCGUAGACACUAUGAGGGAGUUGAGCUGGAUACUUACACCAUGAUCUCAUACGGAGACGACAUCGUGGUGGCAAGUGACUACGAUUUGGACUUUGAGGCUCUCAAGCCCCACUUUAAAUCUCUCGGUCAAACCAUUACUCCAGCUGACAAAAGCGACAAAGGUUUUGUUCUCGGACAUUCCAUCACCGAUGUCACUUUCCUCAAAAGACACUUCCACAUGGAUUAUGGAACUGGGUUUUACAAACCUGUGAUGGCCUCAAAGACUCUCGAGGCCAUUCUCUCCUUUGCACGCCGUGGGACCAUACAGGAGAAGUUGAUCUCCGUGGCAGGACUCGCCGUCCACUCCGGACCAGACGAGUACCGGCGUCUCUUUGAACCCUUCCAGGGCCUCUUUGAGAUUCCAAGCUACAGAUCACUUUACCUGCGUUGGGUGAACGCCGUGUGCGGUGACGCAUAAUCCCUCAGAGAUCACAAUUGGCACAAUGACUCUGGGGCGCGCGACGCCGUAGGAGUGAAAAGCCCGAAGGGCUUCUCCCGCUUCCUUAUCCCAAAAAAAAAAAAAAAA
Lig328	RNA	CCCCUCUCCCUCCCCCCCCCCUAACGUUACUGGCCGAAGCCGCUUGGAAUAAGGCCGGUGUGCGUUUGUCUAUAUGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGAAACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGC
Lig232	RNA	UUGAAAGCCGGGGGUGGGAGAUCCGGAUUGCCAGUCUGCUCGAUAUCGCAGGCUGGGUCCGUGACUACCCACUCCCCCUUUCAACGUGAAGGCUACGAUAGUGCCAGGGCGGGUACUGCCGUAAGUGCCACCCCAAAAUAACAACAGACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCUCUCCCUCCCCCCCCCCUAACGUUACUGGCCGAAGCCGCUUGGAAUAAGGCCGGUGUGCGUUUGUCUAUAUGUUAUUUUCCACCAUAUUGCCGUCUUUUGGCAAUGUGAGGGCCCGGAAACCUGGCCCUGUCUUCUUGACGAGCAUUCCUAGGGGUCUUUCCCCUCUCGCCAAAGGAAUGCAAGGUCUGUUGAAUGUCGUGAAGGAAGCAGUUCCUCUGGAAGCUUCUUGAAGACAAACAACGUCUGUAGCGACCCUUUGCAGGCAGCGGAACCCCCCACCUGGCGACAGGUGCCUCUGCGGCCAAAAGCCACGUGUAUAAGAUACACCUGCAAAGGCGGCACAACCCCAGUGCCACGUUGUGAGUUGGAUAGUUGUGGAAAGAGUCAAAUGGCUCUCCUCAAGCGUAUUCAACAAGGGGCUGAAGGAUGCCCAGAAGGUACCCCAUUGUAUGGGAUCUGAUCUGGGGCCUCGGUGCACAUGCUUUACAUGUGUUUAGUCGAGGUUAAAAAACGUCUAGGCCCCCCGAACCACGGGGACGUGGUUUUCCUUUGAAAAACACGAUGAUAAUAUGGCCACAACCAUGGAACAAGAGACUUGCGCGCACUCUCUCACUUUUGAGGAAUGCCCAAAAUGCUCUGCUCUACAAUACCGUAAUGGAUUUUACCUGCUAAAGUAUGAUGAAGAAUGGUACCCAGAGGAGUUAUUGACUGAUGGAGAGGAUGAUGUCUUUGAUCCCGAAUUAGACAUGGAAGUCGUUUUCGAGUUACAGGGCAAUUCCACCUCCUCAGACAAGAAUAACUCCUCCUCGGAAGGCAAUGAAGGUGUGAUCAUCAAUAACUUUUACUCCAACCAAUAUCAAAACUCCAUUGACCUCUCUGCUAAUGCAGCCGGGUCUGACCCACCCAGAACCUACGGUCAAUUUUCGAAUCUCUUUUCGGGCGCAGUGAAUGCCUUUUCUAAUAUGCUUCCAUUGCUAGCUGAUCAAAAUACAGAAGAAAUGGAGAAUCUGUCUGAUCGAGUGUCUCAAGACACUGCCGGCAAUACGGUCACAAACACCCAGUCAACAGUGGGCCGUCUUGUCGGUUAUGGUACCGUUCAUGAUGGAGAGCAUCCGGCAUCAUGUGCUGACACUGCUUCAGAAAAGAUCCUGGCGGUGGAAAGGUACUACACCUUCAAGGUUAAUGAUUGGACAUCAACACAAAAGCCCUUUGAGUACAUCCGCAUUCCCCUUCCUCACGUCCUGUCCGGUGAAGAUGGUGGUGUCUUUGGUGCGGCCCUCCGCCGGCACUACCUGGUGAAAACUGGAUGGCGGGUGCAAGUUCAGUGCAACGCCUCUCAAUUCCACGCUGGAGGUUUGCUGGUGUUCAUGGCACCAGAGUAUCCAACCUUAGAUGCUUUUGCCAUGGACAACCGUUGGUCCAAGGAUAACCUGCCUAAUGGAACCAGAACUCAGACAAACAAAAAGGGACCAUUUGCCAUGGACCAUCAGAACUUCUGGCAGUGGACCUUGUAUCCCCAUCAAUUCCUGAAUCUGAGAACUAACACCACAGUGGAUCUUGAGGUGCCAUAUGUAAACAUAGCCCCCACUUCCUCCUGGACACAACAUGCUUCCUGGACUUUGGUGAUUGCAGUGGUUGCUCCCCUGACAUACUCAACCGGGGCUUCUACCAGUUUGGAUAUCACCGCUUCUAUUCAGCCAGUAAGGCCUGUCUUUAAUGGCCUCCGGCAUGAGACACUUUCUAGACAGUCGCCCAUUCCGGUCACAAUUAGAGAACAUGCUGGUACCUGGUAUUCUACUCUGCCAGACAGCACAGUGCCUAUUUAUGGCAAGACUCCUGUUGCUCCAUCCAAUUACAUGGUAGGCGAAUACAAGGACUUCCUGGAGAUAGCUCAGAUUCCAACCUUUAUUGGGAAUAAGAUCCCUAAUGCUGUCCCCUACAUUGAGGCAUCCAACACAGCCGUCAAGACCCAACCGCUGGCCACCUAUCAAGUGACCUUGUCCUGCUCCUGUCUGGCCAAUACAUUCUUGGCCGCUUUGUCUAGAAACUUUGCUCAGUACCGGGGAUCAUUGGUUUAUACCUUUGUGUUCACUGGGACCGCGAUGAUGAAGGGCAAGUUCCUCAUUGCCUACACCCCACCUGGAGCGGGCAAGCCCACUAGUCGAGACCAAGCCAUGCAGGCGACUUAUGCGAUUUGGGAUUUGGGGCUAAAUUCUUCUUACUCCUUCACUGUGCCUUUUAUUUCUCCCACUCACUUCCGCAUGGUAGGUACUGACCAAGUCAACAUCACUAAUGCGGAUGGCUGGGUUACCGUGUGGCAGCUCACUCCCCUCACUUACCCACCAGGAUGCCCGACCUCUGCUAAGAUACUAACAAUGGUGAGCGCAGGGAAGGAUUUCUCACUCAAGAUGCCUAUCUCACCUGCCCCCUGGAGCCCUCAGGGAGUAGAAAACGCUGAAAAAGGGGUCACUGAAAACACAAACGCAACUGCUGACUUUGUGGCUCAACCAGUUUACUUGCCUGAGAACCAAACGAAGGUGGCUUUCUUCUAUAAUAGGUCCAGUCCCAUUGGUGCCUUCACCGUGAAGUCCGGCAGUCUAGAAUCUGGUUUUGCCCCGUUCUCUAAUGGGACUUGCCCGAACUCAGUGAUACUGACCCCUGGGCCCCAAUUUGACCCCGCCUAUGACCAACUCAGGCCACAGCGUCUGACAGAAAUUUGGGGCAAUGGAAAUGAGGAGACCUCAAAAGUCUUUCCGCUUAAAUCCAAACAGGAUUAUUCCUUCUGCCUCUUCUCCCCCUUUGUGUAUUAUAAAUGUGAUUUAGAAGUGACUCUUAGUCCUCACACUUCAGGCAACCAUGGGCUGUUGGUGAGGUGGUGUCCCACUGGUACACCAACCAAGCCCACUACCCAGGUUCUCCAUGAAGUAAGUUCCCUCUCAGAAGGCAGAACCCCCCAGGUUUAUAGUGCCGGACCUGGCAUUUCAAAUCAGAUUUCCUUUGUAGUUCCUUACAAUUCUCCACUUUCAGUCCUAUCAGCUGUCUGGUAUAAUGGACACAAGAGAUUUGACAACACUGGGAGCUUGGGCAUUGCCCCUAAUUCUGAUUUCGGCACUCUGUUCUUUGCUGGCACAAAGCCUGACAUUAAAUUCACAGUCUACUUGAGAUACAAGAAUAAGAGAGUUUUUUGCCCACGUCCGACUGUCUUUUUCCCCUGGCCCACUUCCGGAGACAAGAUUGAUAUGACCCCGAGAGCUGGAGUCUUGAUGCUAGAGAGUCCAAAUGCCCUAGACAUUUCAAGAACAUACCCCACGUUACAUGUUCUCAUUCAAUUCAACCAUAGAGGUUUGGAGGUUAGAUUGUUUAGACAUGGACACUUUUGGGCUGAAACACGUGCGGACGUGAUUCUGAGAUCAAAGACCAAACAGGUCUCUUUCCUGAGCAACGGGAACUACCCGUCAAUGGACUCUAGAGCUCCCUGGAAUCCUUGGAAGAAUACCUACCAGGCGGUUCUAAGAGCAGAACCAUGUAGAGUGACCAUGGAUAUAUAUUAUAAGAGAGUCAGGCCUUUUAGACUGCCCCUGGUUCAGAAGGAAUGGCCCGUGCGAGAGGAGAACGUUUUCGGUUUGUACCGGAUCUUCAAUGCCCACUACGCUGGUUACUUUGCGGACCUACUGAUUCAUGACAUUGAGACAAAUCCAGGGCCCUUCAUGUUUAGACCAAGGAAACAGGUUUUCCAGACCCAAGGAGCGGCAGUGUCAUCAAUGGCUCAAACCCUACUGCCGAACGACCUUGCCAGCAAAGCUAUGGGAUCAGCUUUUACGGCUUUGCUCGAUGCCAACGAGGACGCCCAAAAAGCAAUGAAGAUUAUAAAGACAUUAAGUUCUCUAUCGGAUGCAUGGGAAAAUGUAAAAGAAACACUAAACAACCCAGAGUUCUGGAAGCAGCUCUUGAGCAGAUGUGUGCAGCUGAUUGCAGGGAUGACAAUAGCAGUGAUGCAUCCGGACCCUUUGACUCUGCUCUGCUUAGGAACAUUGACGGCCGCCGAGAUUACAAGCCAGACAAGUCUGUGCGAAGAAAUAGCAGCUAAGUUCAAGACAAUUUUCAUCACUCCUCCACCACGGUUUCCCACAAUCUCUCUUUUCCAACAACAAUCCCCCUUGAAACAGGUAAAUGAUAUUUUCUCCCUAGCCAAGAACCUGGACUGGGCCGUCAAGACUGUGGAAAAGGUGGUUGAUUGGUUUGGGACAUGGAUAGUACAGGAGGAAAAGGAACAGACCCUAGAUCAGCUCUUGCAGCGUUUCCCCGAACAUGCGAAGCGCAUUUCUGAUCUCCGGAAUGGAAUGGCCGCCUAUGUAGAGUGCAAGGAGAGUUUUGAUUUCUUUGAAAAGCUGUACAAUCAGGCAGUGAAAGAGAAGAGAACGGGUAUCGCCGCCGUCUGUGAAAAAUUCAGACAGAAGCAUGACCACGCCACCGCUCGGUGUGAGCCAGUCGUGAUUGUGCUCCGCGGAGACGCGGGGCAAGGGAAAUCUUUAUCAAGUCAGGUUAUUGCCCAGGCCGUCUCCAAGACCAUUUUCGGCCGGCAAUCUGUGUAUUCCCUUCCCCCCGAUUCGGAUUUCUUUGAUGGCUAUGAAAAUCAGUUUGCAGCAAUAAUGGAUGAUCUAGGGCAAAAUCCUGAUGGCUCUGAUUUCACUACGUUCUGUCAGAUGGUUUCGACUACCAAUUUUCUCCCCAAUAUGGCUAGUCUAGAGAGAAAGGGCACCCCCUUUACAUCUCAGCUUGUGGUGGCAACUACCAAUCUGCCUGAGUUUAGACCUGUCACAAUAGCCCAUUACCCUGCUGUUGAGAGAAGGAUAACUUUCGACUAUUCAGUGUCUGCUGGUCCAGUCUGCUCCAAAACAGAGGCCGGGUAUAAGGUUUUGGAUGUUGAAAGAGCCUUUAGGCCUACCGGUGAGGCUCCUCUUCCAUGCUUCCAGAAUAACUGCCUUUUCCUUGAGAAAGCUGGGCUCCAGUUCAGAGAUAACCGAACUAAAGAGAUCAUUUCCCUGGUAGAUGUGAUUGAGAGAGCCGUGGCUAGGAUUGAAAGGAAGAAGAAAGUUCUCACAACCGUGCAGACCCUUGUGGCACAAGGUCCAGUAGACGAGGUCAGUUUCCAUUCCGUAGUCCAGCAGCUUAAAGCAAGACAGCAAGCGACAGAUGAACAGCUUGAGGAAUUGCAAGAGGCUUUCGCGAAAGUACAGGAGCGUAACUCUGUGUUUUCUGAUUGGUUGAAGAUUUCUGCAAUGUUGUGUGCUGCGACUCUGGCACUUUCCCAAGUUGUCAAGAUGGCCAAGGCGGUGAAGCAGAUGGUCAAGCCUGAUCUGGUUCGUGUGCAAUUGGAUGAGCAGGAGCAGGGACCUUACAAUGAGACAGCGAGAGUUAAACCAAAAACACUGCAGUUGUUGGACAUUCAGGGACCAAACCCUGUGAUGGACUUUGAAAAAUAUGUAGCCAAACAUGUAACCGCCCCCAUUGGUUUUGUCUACCCCACUGGGGUGAGCACCCAGACUUGCCUCCUUGUGAGAGGCCGCACCUUGGUAGUAAAUAGACACAUGGCCGAGUCUGACUGGACUUCCAUAGUAGUGCGUGGAGUCACACACGCCCGCUCUACUGUUAAAAUUUUGGCCAUAGCUAAAGCAGGCAAAGAGACUGACGUAUCUUUCAUCCGCCUCUCUUCUGGUCCUCUAUUCAGAGACAAUACAUCCAAAUUUGUCAAGGCUGGUGAUGUACUUCCUACUGGUGCCGCUCCAGUCACGGGGAUAAUGAACACGGACAUACCCAUGAUGUACACAGGAACCUUCCUGAAAGCUGGUGUGUCAGUCCCAGUGGAAACCGGCCAGACCUUUAAUCACUGUAUUCAUUACAAGGCUAACACACGAAAGGGCUGGUGUGGAUCAGCCCUACUGGCAGAUCUUGGAGGAAGCAAGAAAAUCCUUGGCAUCCAUUCUGCUGGCUCUAUGGGAAUAGCCGCCGCCUCGAUUGUGUCACAGGAGAUGAUUCGGGCGGUAGUGAAUGCCUUUGAGCCACAGGGUGCUCUCGAGAGAUUGCCAGAUGGGCCCCGUAUUCACGUACCACGUAAAACAGCACUACGCCCCACCGUUGCCCGUCAAGUCUUCCAACCAGCAUAUGCCCCGGCUGUUCUAUCGAAAUUUGACCCUAGAACAGAGGCUGAUGUAGAUGAAGUGGCUUUCUCCAAACAUACCUCCAACCAGGAAAGCCUCCCACCAGUGUUUAGAAUGGUAGCCAAAGAGUAUGCCAAUAGAGUUUUCACCUUGCUGGGAAAAGACAAUGGCCGUCUGACUGUAAAGCAGGCUUUGGAAGGACUGGAGGGGAUGGACCCCAUGGACAGGAACACCUCCCCGGGGCUUCCAUAUACUGCGCUAGGAAUGCGCAGAACAGAUGUCGUAGAUUGGGAAUCAGCCACCCUGAUCCCGUUUGCGGCAGAAAGAUUAAGAAAAAUGAAUGAAGGAGACUUUUCCGAAGUUGUCUAUCAAACAUUCCUCAAGGAUGAGCUUAGACCGAUAGAGAAGGUUCAAGCCGCCAAGACACGGAUUGUAGAUGUUCCACCAUUUGAGCAUUGCAUUCUGGGUAGACAAUUGUUGGGAAAGUUUGCAUCAAAGUUCCAGACCCAACCGGGUCUGGAACUAGGAUCAGCCAUUGGAUGUGACCCAGAUGUACACUGGACUGCCUUCGGUGUCGCCAUGCAAGGUUUUGAGCGUGUCUACGAUGUGGACUACUCCAACUUUGAUUCGACCCAUUCGGUGGCAAUGUUCCGCUUAUUGGCUGAGGAAUUUUUCACUCCAGAGAAUGGUUUUGACCCCCUGACUAGAGAAUAUCUUGAGUCAUUAGCCAUUUCAACCCAUGCGUUUGAGGAGAAGCGCUUUCUGAUAACCGGUGGUCUCCCAUCAGGUUGUGCAGCGACCUCAAUGCUAAACACUAUAAUGAAUAAUAUAAUAAUUAGGGCGGGUUUGUAUCUCACGUAUAAAAAUUUUGAAUUUGAUGAUGUGAAGGUGUUGUCGUACGGAGAUGAUCUCCUUGUGGCCACAAAUUACCAAUUGGAUUUUGAUAAGGUGAGAGCAAGCCUCGCAAAGACAGGAUAUAAGAUAACUCCCGCUAACACAACUUCUACCUUUCCUCUUAAUUCGACGCUUGAAGACGUUGUCUUCUUAAAAAGAAAGUUUAAGAAAGAGGGCCCUCUGUAUCGGCCUGUCAUGAACAGAGAGGCGUUGGAAGCAAUGUUGUCAUACUAUCGUCCAGGGACUCUAUCUGAGAAACUCACUUCGAUCACUAUGCUUGCCGUUCAUUCUGGCAAGCAGGAAUAUGAUCGGCUCUUUGCCCCAUUCCGUGAGGUAGGGGUUGUCGUGCCAUCAUUCGAGAGUGUGGAGUACAGAUGGAGGAGUCUGUUCUGGUAGUAGUGUAGUCACUGGCACAACGCGUUACCCGGUAAGCCAAUCGGGUAUACACGGUCGUCAUACUGCAGACAGGGUUCUUCUACUUUGCAAGAUAGUCUAGAGUAGUAAAAUAAAUAGAUAGAG
Lig223	RNA	CUCUCU