U4/U6 snRNA-associated-splicing factor PRP24

Protein information

  • Protein ID: P49960
  • Protein Name : U4/U6 snRNA-associated-splicing factor PRP24
  • Gene : PRP24
  • Organism : Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
  • Status : reviewed
  • Function : Binds preferentially to the U4/U6 hybrid snRNAs. Can stimulate the annealing of U4 and U6. Could participate in both the formation and disassembly of the U4/U6 hybrid during splicing.

Protein Sequence

  • MEYGHHARPDSKRPLDEGSPAAAGLTSKKANEALTRNRELTTVLVKNLPKSYNQNKVYKYFKHCGPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHKVVGQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLSTELLDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCAFMVFENKDSAERALQMNRSLLGNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDMKRYYNNQQNHSMKHVKPSCINMMEKGPNLQVKKKIPDKQEQMSNDDFRKMFLGE

RRM domains in this Protein - 4 

RRM Entryname RRM Sequence range Pfam ID
P49960_RRM1 43 - 110 PF00076
P49960_RRM2 119 - 189 PF00076
P49960_RRM3 212 - 283 PF00076
P49960_RRM4 311 - 393 PF16842

RRM domain Linkers in this Protein - 3 

Linker Name Linker Sequence range
P49960_linker1 111 - 118
P49960_linker2 190 - 211
P49960_linker3 284 - 310

Experiments retrieved for this Protein - 8 

Experiment ID Experiment Type PubMed ID
Exp_2GHP X-ray Diffraction 17320109
Exp_2GO9 NMR 17320109
Exp_2KH9 NMR 20181740
Exp_2L9W NMR 21653550
Exp_4N0T X-ray Diffraction 24837192
Exp_5TF6 X-ray Diffraction 28045380
Exp_5VSU X-ray Diffraction 29717126
Exp_6ASO X-ray Diffraction 29717126

Ligands tested for Binding - 14 

Ligand ID Ligand Type Ligand Sequence
Lig128 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC
Lig123 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUX
Lig128 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC
Lig202 RNA AGAGAU
Lig212 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGGUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUUU
Lig56 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC
Lig123 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUX
Lig128 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC
Lig212 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGGUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUUU
Lig56 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC
Lig123 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUX
Lig128 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC
Lig212 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGGUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUUU
Lig56 RNA GGUCAAUUUGAAACAAUACAGAGAUGAUCAGCGGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGACC