TAR DNA-binding protein 43

Protein information

  • Protein ID: Q13148
  • Protein Name : TAR DNA-binding protein 43
  • Gene : TARDBP
  • Organism : Homo sapiens
  • Status : reviewed
  • Function : RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds via its two RNA recognition motifs RRM1 and RRM2 to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609 PubMed:24240615 PubMed:24464995). In turn regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640 PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:23398327 PubMed:19765185). Participates also in the normal skeletal muscle formation and regeneration forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6 ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).

Protein Sequence

  • MSEYIRVTEDENDEPIEIPSEDDGTVLLSTVTAQFPGACGLRYRNPVSQCMRGVRLVEGILHAPDAGWGNLVYVVNYPKDNKRKMDETDASSAVKVKRAVQKTSDLIVLGLPWKTTEQDLKEYFSTFGEVLMVQVKKDLKTGHSKGFGFVRFTEYETQVKVMSQRHMIDGRWCDCKLPNSKQSQDEPLRSRKVFVGRCTEDMTEDELREFFSQYGDVMDVFIPKPFRAFAFVTFADDQIAQSLCGEDLIIKGISVHISNAEPKHNSNRQLERSGRFGGNPGGFGNQGGFGNSRGGGAGLGNNQGSNMGGGMNFGAFSINPAMMAAAQAALQSSWGMMGMLASQQNQSGPSGNNQNQGNMQREPNQAFGSGNNSYSGSNSGAAIGWGSASNAGSGSGFNGGFGSSMDSKSSGWGM

RRM domains in this Protein - 2 

RRM Entryname RRM Sequence range Pfam ID
Q13148_RRM1 106 - 172 PF00076
Q13148_RRM2 193 - 243 PF00076

RRM domain Linkers in this Protein - 1 

Linker Name Linker Sequence range
Q13148_linker1 173 - 192

Experiments retrieved for this Protein - 25 

Experiment ID Experiment Type PubMed ID
Exp_310 ITC 24240615
Exp_311 ITC 24240615
Exp_312 ITC 24240615
Exp_313 ITC 24240615
Exp_314 ITC 24240615
Exp_315 ITC 24240615
Exp_316 ITC 24240615
Exp_317 ITC 24240615
Exp_318 ITC 24240615
Exp_319 ITC 24240615
Exp_320 ITC 24240615
Exp_321 ITC 24240615
Exp_322 ITC 24240615
Exp_323 ITC 24240615
Exp_324 ITC 24240615
Exp_325 ITC 24240615
Exp_326 ITC 24240615
Exp_327 ITC 24240615
Exp_328 ITC 24240615
Exp_1WF0 NMR
Exp_2CQG NMR
Exp_4BS2 NMR 24240615
Exp_4IUF X-ray Diffraction 24464995
Exp_4Y00 X-ray Diffraction 26883171
Exp_4Y0F X-ray Diffraction 26883171

Ligands tested for Binding - 12 

Ligand ID Ligand Type Ligand Sequence
Lig106 DNA GTTGAGCGTT
Lig121 RNA GUGUGAAUGAAU
Lig261 RNA GUGUGAUGAAU
Lig272 RNA GUGUGUGAAUGAAU
Lig296 RNA GUGUGUGUGUGU
Lig340 RNA GUGUGUGUGAAU
Lig79 DNA GTTGAGCGT
Lig121 RNA GUGUGAAUGAAU
Lig261 RNA GUGUGAUGAAU
Lig272 RNA GUGUGUGAAUGAAU
Lig296 RNA GUGUGUGUGUGU
Lig340 RNA GUGUGUGUGAAU