Cleavage and polyadenylation specificity factor subunit 6

Protein information

  • Protein ID: Q16630
  • Protein Name : Cleavage and polyadenylation specificity factor subunit 6
  • Gene : CPSF6
  • Organism : Homo sapiens
  • Status : reviewed
  • Function : Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs (PubMed:9659921 PubMed:8626397 PubMed:14690600 PubMed:29276085). CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end so called cleavage and polyadenylation signals (pA signals) (PubMed:9659921 PubMed:8626397 PubMed:14690600). Most pre-mRNAs contain multiple pA signals resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation (PubMed:23187700 PubMed:29276085). The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs (PubMed:20695905 PubMed:29276085). CPSF6 enhances NUDT21/CPSF5 binding to 5'-UGUA-3' elements localized upstream of pA signals and promotes RNA looping and hence activates directly the mRNA 3'-processing machinery (PubMed:15169763 PubMed:29276085 PubMed:21295486). Plays a role in mRNA export (PubMed:19864460).

Protein Sequence

  • MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNRNRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSRRERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR

RRM domains in this Protein - 1 

RRM Entryname RRM Sequence range Pfam ID
Q16630_RRM1 83 - 154 PF00076

Experiments retrieved for this Protein - 4 

Experiment ID Experiment Type PubMed ID
Exp_3P5T X-ray Diffraction
Exp_3P6Y X-ray Diffraction
Exp_3Q2S X-ray Diffraction 21295486
Exp_3Q2T X-ray Diffraction 21295486

Ligands tested for Binding - 2 

Ligand ID Ligand Type Ligand Sequence
Lig175 RNA UGUAA
Lig198 RNA UUGUA