Q389P7_RRM1

Protein information

  • Protein ID: Q389P7
  • Protein Name: RNA-binding protein putative
  • Organism: Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
  • Gene Name: Tb10.406.0050

Domain information

  • Pfam ID: PF00076
  • Pfam Clan Name: RRM
  • Domain Range: 194 - 256
  • Domain Length: 63

Domain Sequence

  • VQVSGLSDETTWHTLKDHLRQAGEVTFCKVFSGGRAVVEFVTPEDAARAITELQASELEGATL

Available Structures for this domain - 14 

Experiment ID RRM Structure Experiment Type Ligand ID Contacts
Exp_6E4N 6E4NA01 X-ray Diffraction No contacts
Exp_6E4O 6E4OA01 X-ray Diffraction No contacts
Exp_6E4O 6E4OB01 X-ray Diffraction No contacts
Exp_6E4O 6E4OC01 X-ray Diffraction No contacts
Exp_6E4O 6E4OD01 X-ray Diffraction No contacts
Exp_6E4P 6E4PA01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PB01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PC01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PD01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PE01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PF01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PG01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PH01 X-ray Diffraction Lig167 Contacts
Exp_6E4P 6E4PI01 X-ray Diffraction Lig167 Contacts

Binding Experiments for this domain - 22 

Experiment ID RRM Entryname Experiment Type Ligand ID Binds? Binding Affinity
Exp_1 Q389P7_RRM1 Fluorescence polarization Lig279 Yes 1.4 ± 0.03 μM
Exp_2 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 27 ± 11 nM
Exp_3 Q389P7_RRM1 Fluorescence polarization Lig342 Yes 6.3 ± 1.4 μM
Exp_4 Q389P7_RRM1 Fluorescence polarization Lig253 No
Exp_5 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 50 ± 4 nM
Exp_6 Q389P7_RRM1 Fluorescence polarization Lig279 No
Exp_7 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 39 ± 4 nM
Exp_8 Q389P7_RRM1 Fluorescence polarization Lig279 No
Exp_9 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 157 ± 39 nM
Exp_10 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 442 ± 43 nM
Exp_11 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 16 ± 4 nM
Exp_12 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 11 ± 3 nM
Exp_13 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 21 ± 4 nM
Exp_14 Q389P7_RRM1 Fluorescence polarization Lig338 Yes 1.9 ± 0.7 nM
Exp_15 Q389P7_RRM1 Fluorescence polarization Lig279 Yes 240 ± 37 nM
Exp_16 Q389P7_RRM1 Fluorescence polarization Lig342 Yes 1 ± 0.1 μM
Exp_17 Q389P7_RRM1 Fluorescence polarization Lig253 Yes 2.6 ± 0.4 μM
Exp_18 Q389P7_RRM1 Fluorescence polarization Lig254 Yes 28 ± 6 nM
Exp_19 Q389P7_RRM1 Fluorescence polarization Lig254 Yes 156 ± 24 nM
Exp_20 Q389P7_RRM1 Fluorescence polarization Lig254 Yes 163 ± 47 nM
Exp_21 Q389P7_RRM1 Fluorescence polarization Lig279 No
Exp_22 Q389P7_RRM1 Fluorescence polarization Lig279 No

Ligands tested for Binding - 6 

Ligand ID Ligand Type Ligand Sequence
Lig167 RNA UUUU
Lig253 RNA CCCCCCCCCCCCCCCCCCCC
Lig254 RNA UAGGGUUAGGGU
Lig279 RNA UUUUUUUUUUUUUUUUUUUU
Lig338 RNA GGGGGGGGGGGGGGGGGGGG
Lig342 RNA AAAAAAAAAAAAAAAAAAAA