Splicing factor U2AF 65 kDa subunit
Protein information
- Status : reviewed
- Function : Plays a role in pre-mRNA splicing and 3'-end processing (PubMed:17024186). By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD) and thereby pre-mRNA may couple transcription to splicing (PubMed:21536736). Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. Positively regulates pre-mRNA 3'-end processing by recruiting the CFIm complex to cleavage and polyadenylation signals (PubMed:17024186).
Protein Sequence
- MSDFDEFERQLNENKQERDKENRHRKRSHSRSRSRDRKRRSRSRDRRNRDQRSASRDRRRRSKPLTRGAKEEHGGLIRSPRHEKKKKVRKYWDVPPPGFEHITPMQYKAMQAAGQIPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLVSPPSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW
RRM domains in this Protein - 3
| RRM Entryname | RRM Sequence range | Pfam ID |
|---|---|---|
| P26368_RRM1 | 151 - 225 | PF00076 |
| P26368_RRM2 | 261 - 331 | PF00076 |
| P26368_RRM3 | 399 - 460 | PF00076 |
RRM domain Linkers in this Protein - 2
| Linker Name | Linker Sequence range |
|---|---|
| P26368_linker1 | 226 - 260 |
| P26368_linker2 | 332 - 398 |
Experiments retrieved for this Protein - 31
| Experiment ID | Experiment Type | PubMed ID |
|---|---|---|
| Exp_1O0P | NMR | 12718882 |
| Exp_1OPI | NMR | 12718882 |
| Exp_1U2F | NMR | 10449418 |
| Exp_2G4B | X-ray Diffraction | 16818232 |
| Exp_2HZC | X-ray Diffraction | 17188295 |
| Exp_2M0G | NMR | 23175611 |
| Exp_2U2F | NMR | 10449418 |
| Exp_2YH0 | NMR | 21753750 |
| Exp_2YH1 | NMR | 21753750 |
| Exp_3VAF | X-ray Diffraction | 23376934 |
| Exp_3VAG | X-ray Diffraction | 23376934 |
| Exp_3VAH | X-ray Diffraction | 23376934 |
| Exp_3VAI | X-ray Diffraction | 23376934 |
| Exp_3VAJ | X-ray Diffraction | 23376934 |
| Exp_3VAK | X-ray Diffraction | 23376934 |
| Exp_3VAL | X-ray Diffraction | 23376934 |
| Exp_3VAM | X-ray Diffraction | 23376934 |
| Exp_4FXW | X-ray Diffraction | 23273425 |
| Exp_4TU7 | X-ray Diffraction | 25422459 |
| Exp_4TU8 | X-ray Diffraction | 25422459 |
| Exp_4TU9 | X-ray Diffraction | 25422459 |
| Exp_5EV1 | X-ray Diffraction | 26952537 |
| Exp_5EV2 | X-ray Diffraction | 26952537 |
| Exp_5EV3 | X-ray Diffraction | 26952537 |
| Exp_5EV4 | X-ray Diffraction | 26952537 |
| Exp_5W0G | X-ray Diffraction | 28850223 |
| Exp_5W0H | X-ray Diffraction | 28850223 |
| Exp_6TR0 | NMR | 32188783 |
| Exp_6XLV | X-ray Diffraction | 33020180 |
| Exp_6XLW | X-ray Diffraction | 33020180 |
| Exp_6XLX | X-ray Diffraction | 33020180 |
Ligands tested for Binding - 24
| Ligand ID | Ligand Type | Ligand Sequence |
|---|---|---|
| Lig108 | RNA | UUUUUCU |
| Lig113 | RNA | UCUUUUU |
| Lig15 | RNA | UUUUUUUUU |
| Lig150 | RNA | UUUCUUU |
| Lig152 | RNA | UUUUUUUU |
| Lig185 | RNA | UUUUUUCC |
| Lig203 | RNA | UUUUUUUUC |
| Lig208 | RNA | UUUUCUU |
| Lig213 | RNA | UUUUUGU |
| Lig54 | RNA | UUUUUUU |
| Lig55 | RNA | CUUUUUU |
| Lig61 | RNA | UUUUUAU |
| Lig108 | RNA | UUUUUCU |
| Lig113 | RNA | UCUUUUU |
| Lig15 | RNA | UUUUUUUUU |
| Lig150 | RNA | UUUCUUU |
| Lig152 | RNA | UUUUUUUU |
| Lig185 | RNA | UUUUUUCC |
| Lig203 | RNA | UUUUUUUUC |
| Lig208 | RNA | UUUUCUU |
| Lig213 | RNA | UUUUUGU |
| Lig54 | RNA | UUUUUUU |
| Lig55 | RNA | CUUUUUU |
| Lig61 | RNA | UUUUUAU |