U1 small nuclear ribonucleoprotein A

Protein information

  • Protein ID: P09012
  • Protein Name : U1 small nuclear ribonucleoprotein A
  • Gene : SNRPA
  • Organism : Homo sapiens
  • Status : reviewed
  • Function : Component of the spliceosomal U1 snRNP which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. U1 snRNP is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. SNRPA binds stem loop II of U1 snRNA. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. May bind preferentially to the 5'-UGCAC-3' motif on RNAs.

Protein Sequence

  • MAVPETRPNHTIYINNLNEKIKKDELKKSLYAIFSQFGQILDILVSRSLKMRGQAFVIFKEVSSATNALRSMQGFPFYDKPMRIQYAKTDSDIIAKMKGTFVERDRKREKRKPKSQETPATKKAVQGGGATPVVGAVQGPVPGMPPMTQAPRIMHHMPGQPPYMPPPGMIPPPGLAPGQIPPGAMPPQQLMPGQMPPAQPLSENPPNHILFLTNLPEETNELMLSMLFNQFPGFKEVRLVPGRHDIAFVEFDNEVQAGAARDALQGFKITQNNAMKISFAKK

RRM domains in this Protein - 2 

RRM Entryname RRM Sequence range Pfam ID
P09012_RRM1 12 - 83 PF00076
P09012_RRM2 210 - 275 PF00076

RRM domain Linkers in this Protein - 1 

Linker Name Linker Sequence range
P09012_linker1 84 - 209

Experiments retrieved for this Protein - 85 

Experiment ID Experiment Type PubMed ID
Exp_1AUD NMR 9312034
Exp_1DRZ X-ray Diffraction 9783582
Exp_1DZ5 NMR 10742179
Exp_1FHT NMR 8609632
Exp_1M5K X-ray Diffraction 11298439
Exp_1M5O X-ray Diffraction 12376595
Exp_1M5P X-ray Diffraction 12376595
Exp_1M5V X-ray Diffraction 12376595
Exp_1NU4 X-ray Diffraction 12876372
Exp_1OIA X-ray Diffraction 2147232
Exp_1SJ3 X-ray Diffraction 15141216
Exp_1SJ4 X-ray Diffraction 15141216
Exp_1SJF X-ray Diffraction 15141216
Exp_1U6B X-ray Diffraction 15175762
Exp_1URN X-ray Diffraction 7984237
Exp_1VBX X-ray Diffraction 15141216
Exp_1VBY X-ray Diffraction 15141216
Exp_1VBZ X-ray Diffraction 15141216
Exp_1VC0 X-ray Diffraction 15141216
Exp_1VC5 X-ray Diffraction 15141216
Exp_1VC6 X-ray Diffraction 15141216
Exp_1ZZN X-ray Diffraction 16141079
Exp_2A3J NMR 16698546
Exp_2NZ4 X-ray Diffraction 17196404
Exp_2OIH X-ray Diffraction 17355864
Exp_2OJ3 X-ray Diffraction 17355864
Exp_2U1A NMR 9265619
Exp_3BO2 X-ray Diffraction 18408159
Exp_3BO3 X-ray Diffraction 18408159
Exp_3BO4 X-ray Diffraction 18408159
Exp_3CUL X-ray Diffraction 18548004
Exp_3CUN X-ray Diffraction 18548004
Exp_3EGZ X-ray Diffraction 18940672
Exp_3G8S X-ray Diffraction 19228039
Exp_3G8T X-ray Diffraction 19228039
Exp_3G96 X-ray Diffraction 19228039
Exp_3G9C X-ray Diffraction 19228039
Exp_3HHN X-ray Diffraction 19965478
Exp_3IIN X-ray Diffraction 20145044
Exp_3IRW X-ray Diffraction 19898477
Exp_3IWN X-ray Diffraction 19898478
Exp_3K0J X-ray Diffraction 19948769
Exp_3L3C X-ray Diffraction 19228039
Exp_3MUM X-ray Diffraction 20690679
Exp_3MUR X-ray Diffraction 20690679
Exp_3MUT X-ray Diffraction 20690679
Exp_3MUV X-ray Diffraction 20690679
Exp_3MXH X-ray Diffraction 20690679
Exp_3P49 X-ray Diffraction 21439473
Exp_3PGW X-ray Diffraction 21113136
Exp_3R1H X-ray Diffraction 21857665
Exp_3R1L X-ray Diffraction 21857665
Exp_3UCU X-ray Diffraction 22148472
Exp_3UCZ X-ray Diffraction 22148472
Exp_3UD3 X-ray Diffraction 22148472
Exp_3UD4 X-ray Diffraction 22148472
Exp_4C4W X-ray Diffraction 24482444
Exp_4PR6 X-ray Diffraction 25326328
Exp_4PRF X-ray Diffraction 25326328
Exp_4W90 X-ray Diffraction 25271255
Exp_4W92 X-ray Diffraction 25271255
Exp_4YB1 X-ray Diffraction 25818298
Exp_5DDO X-ray Diffraction 26655897
Exp_5DDP X-ray Diffraction 26655897
Exp_5DDQ X-ray Diffraction 26655897
Exp_5DDR X-ray Diffraction 26655897
Exp_5FJ4 X-ray Diffraction 27016741
Exp_6LAS X-ray Diffraction 31844059
Exp_6LAU X-ray Diffraction 31844059
Exp_6LAX X-ray Diffraction 31844059
Exp_6LAZ X-ray Diffraction 31844059
Exp_6QX9 EM 30975767
Exp_6SQN X-ray Diffraction 32070773
Exp_6SQQ X-ray Diffraction 32070773
Exp_6SQT X-ray Diffraction 32070773
Exp_6SQV X-ray Diffraction 32070773
Exp_6SR7 X-ray Diffraction 32070773
Exp_6XH0 X-ray Diffraction 33051202
Exp_6XH1 X-ray Diffraction 33051202
Exp_6XH2 X-ray Diffraction 33051202
Exp_6XH3 X-ray Diffraction 33051202
Exp_7AEP NMR 33677607
Exp_7B0Y EM 33446560
Exp_7D7V X-ray Diffraction 33170270
Exp_7LHX X-ray Diffraction

Ligands tested for Binding - 35 

Ligand ID Ligand Type Ligand Sequence
Lig10 RNA GGCCGGCAUGGUCCCAGCCUCCUCGCUGGCGCCGGCUGGGCAACACCAUUGCACUCCGGUGGCGAAUGGGACGGCCGGCAUGGUCCCAGCCUCCUCGCUGGCGCCGGCUGGGCAACACCAUUGCACUCCGGUGGCGAAUGGGAC
Lig125 RNA GGCCGGCAUGGUCCCAGCCUCCUCGCUGGCGCCGGCUGGGCAACACCAUUGCACUCCGGUGGCGAAUGGGAC
Lig130 RNA GGUCACGCACAGAGCAAACCAUUCGAAAGAGUGGGACGCAAAGCCUCCGGCCUAAACCAUUGCACUCCGGUAGGUAGCGGGGUUAUCGAUGG
Lig14 RNA GAUGGCCGGCAUGGUCCCAGCCUCCUCGCUGGCGCCGGCUGGGCAACACCAUUGCACUCCGGUGGCGAAUGGGACU
Lig170 RNA GAGACAUUGCACCCGGAGUCUC
Lig171 RNA GGCACCAUUGCACUCCGGUGCCAGUUGACGAGAUGGGGUUUAUCGAGAUUUCGGCGGAUGACUCCCGGUUGUUCAUCACAACCGCAAGCUUUUACUUAAAUCAUUAAGGUGACUUAGUGGACAAAGGUGAAAGUGUGAUGA
Lig172 RNA GGAUGGCGAAAGCCAUUUCCGCAGGCCCCAUUGCACUCCGGGGUAUUGGCGUUAGGUGGUGGUACGAGGUUCGAAUCCUCGUACCGCAGCCA
Lig176 RNA GGCAGAGUCCUUCGGGACAUUGCACCUGCC
Lig186 RNA GGCCGUGUGCCUUGCGCCGGGAAACCACGCAAGGGAUGGUGUCAAAUUCGGCGAAACCUAAGCGCCCGCCCGGGCGUAUGGCAACGCCGAGCCAAGCUUCGCAGCCAUUGCACUCCGGCUGCGAUGAAGGUGUAGAGACUAGACGGCACCCACCUAAGGCAAACGCUAUGGUGAAGGCAUAGUCCAGGGAGUGGCGA
Lig189 RNA GCGCGCUUAAUCUGAAAUCAGAGCGGGGGACCCAUUGCACUCCGGGUUUUUCCCGUAGGGGUGAAUCCUUUUUAGGUAGGGCGAAAGCCCGAAUCCGUCAGCUAACCUCGUAAGCGCGC
Lig192 RNA GAGGGAGAGGUGAAGAAUACGACCACCUAGGUACCAUUGCACUCCGGUACCUAAAACAUACCCUC
Lig194 RNA GGUCACGCACAGAGCAAACCAUUCGAAAGAGUGGGACGCAAAGCCUCCGGCCUAAACCAUUGCACUCCGGUAGGUAGCGGGGUUACCGAUGG
Lig195 RNA CGUUGGCCCAGGAAACUGGGUGGAAGUAAGGUCCAUUGCACUCCGGGCCUGAAGCAACGCU
Lig200 RNA GAUGGCCGGCAUGGUCCCAGCCUCCUCGCUGGCGCCGGCUGGGCAACACCAUUGCACUCCGGUGGUGAAUGGGACU
Lig205 RNA GGAACACUAUACUACUGGAUAAUCAAAGACAAAUCUGCCCGAAGGGUUUGAGAACAUACCCAUUGCACUCCGGGUAUGCAGAGGUGGCAGCCUCCGGUGGGUUAAAACCCAACGUUCUCAACAAUAGUGA
Lig206 RNA GGUCACGCACAGGGCAAACCAUUCGAAAGAGUGGGACGCAAAGCCUCCGGCCUAAACCAUUGCACUCCGGUAGGUAGCGGGGUUAUCGAUGG
Lig215 RNA GAGGGAGCGCCAUUGCACUCCGGUGCGAAGAACUC
Lig219 RNA GCGCAACAUCCCCGCCGGUUGGGCGCAUUGCACUCCGCGCAGUGAACCGGCUGCGCC
Lig222 RNA GGAGAUCUGAGCCUGGGAGCUCUCUCC
Lig25 RNA GCGACUCGGGGUGCCCUCCAUUGCACUCCGGAGGCUGAGAAAUACCCGUAUCACCUGAUCUGGAUAAUGCCAGCGUAGGGAAGUCGC
Lig31 RNA GGGCACGCACAGAGCAAACCAUUCGAAAGAGUGGGACGCAAAGCCUCCGGCCUAAACCAUUGCACCUCGGUAGGUAGCGGGGUUACCGAUG
Lig42 RNA AUACUUACCUGGCAGGGGAGAUACCAUGAUCACGAAGGUGGUUUUCCCAGGGCGAGGCUUAUCCAUUGCACUCCGGAUGUGCUGACCCCUGCGAUUUCCCCAAAUGUGGGAAACUCGACUGCAUAAUUUGUGGUAGUGGGGGACUGCGUUCGCGCUUUCCCCUG
Lig45 RNA GGCAUUGUGCCUCGCAUUGCACUCCGCGGGGCGAUAAGUCCUGAAAAGGGAUGUC
Lig47 RNA GAUGGCCGGCAUGGUCCCAGCCUCCUCGCUGGCGCCGGCUGGGCAACACCAUUGCACUCCGGUGGUGAAUGGGAC
Lig51 RNA AAUCCAUUGCACUCCGGAUUU
Lig57 RNA UCCAGUAGGAACACUAUACUACUGGAUAAUCAAAGACAAAUCUGCCCGAAGGGCUUGAGAACAUACCCAUUGCACUCCGGGUAUGCAGAGGUGGCAGCCUCCGGUGGGUUAAAACCCAACGUUCUCAACAAUAGUGA
Lig6 RNA GCAGAUCUGAGCCUGGGAGCUCUCUGC
Lig60 RNA GGAGAGAGAAGUCAACCAGAGAAACACACCAACCCAUUGCACUCCGGGUUGGUGGUAUAUUACCUGGUACGGGGGAAACUUCGUGGUGGCCG
Lig66 RNA GAGCAAGAGCCAUUGCACUCCGGUUUGAUGACCUC
Lig68 RNA CACGCACAGGGCAAACCAUUCGAAAGAGUGGGACGCAAAGCCUCCGGCCUAAACGGCAUUGCACUCCGCCGUAGGUAGCGGGGUUACCGAUGG
Lig74 RNA GGUCACGCACAGGGCAAACCAUUCGAAAGAGUGGGACGCAAAGCCUCCGGCCUAAACCAUUGCACUCCGGUAGGUAGCGGGGUUACCGAUGG
Lig8 RNA GGCAUCGUGCCUCGCAUUGCACUCCGCGGGGCGAUAAGUCCUGAAAAGGGAUGUC
Lig84 RNA GGAUAUGAGGAGAGAUUUCAUCCAUUGCACUCCGGAUGAAACACCGAAGAAGUAAAUCUUUCAGGUAAAAAGGACUCAUAUUGGACGAACCUCUGGAGAGCUUAUCUAAGAGAUAACACCGAAGGAGCAAAGCUAAUUUUAGCCUAAACUCUCAGGUAAAAGGACGGAG
Lig85 RNA CGUUGACCCAGGAAACUGGGCGGAAGUAAGGUCCAUUGCACUCCGGGCCUGAAGCAACGCG
Lig88 RNA GGCACCAUUGCACUCCGGUGCCAGUUGACGAGGUGGGGUUUAUCGAGAUUUCGGCGGAUGACUCCCGGUUGUUCAUCACAACCGCAAGCUUUUACUUAAAUCAUUAAGGUGACUUAGUGGACAAAGGUGAAAGUGUGAUGA