Pre-mRNA-splicing factor CWC2

Protein information

  • Protein ID: Q12046
  • Protein Name : Pre-mRNA-splicing factor CWC2
  • Gene : CWC2
  • Organism : Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
  • Status : reviewed
  • Function : Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1 U4 U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the 'nineteen complex' NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Binds also to U1 U4 U5 and U6 snRNAs and to pre-mRNAs in vitro. Is not required for the Prp2-mediated remodeling of the activated spliceosome.

Protein Sequence

  • MTSWRDKSAKVQVKESELPSSIPAQTGLTFNIWYNKWSQGFAGNTRFVSPFALQPQLHSGKTRGDNDGQLFFCLFFAKGMCCLGPKCEYLHHIPDEEDIGKLALRTEVLDCFGREKFADYREDMGGIGSFRKKNKTLYVGGIDGALNSKHLKPAQIESRIRFVFSRLGDIDRIRYVESKNCGFVKFKYQANAEFAKEAMSNQTLLLPSDKEWDDRREGTGLLVKWANEDPDPAAQKRLQEELKLESLNMMVHLINNNTNSAGTEVNNKNNERLDRTFPEASVDNVKKRLLPLDNGMESDDFIEKLKKVKKNISRENISSKPSVGKLGGPLLDYLSSDED

RRM domains in this Protein - 1 

RRM Entryname RRM Sequence range Pfam ID
Q12046_RRM1 149 - 206 PF00076

Experiments retrieved for this Protein - 19 

Experiment ID Experiment Type PubMed ID
Exp_3TP2 X-ray Diffraction 22407296
Exp_3U1L X-ray Diffraction 21957909
Exp_3U1M X-ray Diffraction 21957909
Exp_5GM6 EM 27445306
Exp_5GMK EM 27445308
Exp_5LJ3 EM 27459055
Exp_5LJ5 EM 27459055
Exp_5LQW EM 27562955
Exp_5MPS EM 28076345
Exp_5MQ0 EM 28076345
Exp_5WSG EM 27980089
Exp_5Y88 EM 28919079
Exp_5YLZ EM 29153833
Exp_6BK8 EM 29146870
Exp_6EXN EM 29146871
Exp_6J6G EM 30879786
Exp_6J6H EM 30879786
Exp_6J6N EM 30879786
Exp_6J6Q EM 30879786

Ligands tested for Binding - 10 

Ligand ID Ligand Type Ligand Sequence
Lig105 RNA AAUUUUUAAGGUAUGUAUUUUUUUU
Lig107 RNA GUAUGUCUAAAGUUAUGGCCACGUUUCAAAUGCGUGCUUUUUUUUUAAAACUUAUGCUCUUAUUUACUAACAAAAUCAACAUGCUAUUGAACUAG
Lig116 RNA GAGAGAUUCCGUACACCAUCAGGGUACGAGCUAGCCCAUGGCGUACACCAUCAGGGUACGACUAGUAGAUCUCGUACACCAUCAGGGUACGGAAUUCUCUAGAGUGUCGACGGAUCCCCCUUUUAGAUUUUUCACGCUUACUGCUUUUUUCUUCCCAAGAUCGAAAAUUUACUGAAUUAACAAUGGAUUCUGGUAUGUUCUAGCGCUUGCACCAUCCCAUUUAACUGUAAGAAGAAUUGCACGGUCCCAAUUGCUCGAGAGAUUUCUCUUUUACCUUUUUUUACUAUUUUUCACUCUCCCAUAACCUCCUAUAUUGACUGAUCUGUAAUAACCACGAUAUUAUUGGAAUAAAUAGGGGCUUGAAAUUUGGAAAAAAAAAAAAAACUGAAAUAUUUUCGUGAUAAGUGAUAGUGAUAUUCUUCUUUUAUUUGCUACUGUUACUAAGUCUCAUGUACUAACAUCGAUUGCUUCAUUCUUUUUGUUGCUAUAUUAUAUGUUUAGAGGUUGCUGCUUUGGUUAUUGAUAACGGUUCUGGUAUGUGUAAAGCCGGUUUUGCCGGUGACGACGCUCCUCGUGCUGUCUUCCCAUCUAUCGUCGGUAGACCAAGACACCAAGGUAUCAUGGUCGGUAUGGGUCAAAAAGACUCCUACGUUGGUGAUGAAGCUCAAUCCAAGAGAGG
Lig142 RNA ACGAAUCUCUUUGCCUUUUGGCUUAGAUCAAGUGUAGUAUCUGUUCUUUUCAGUGUAACAACUGAAAUGACCUCAAUGAGGCUCAUUACCUUUUAAUUUGUUACAAUACACAUUUUUUGGCACCCAAAAUAAUAAAAUGGACGGGAAGAGACUUUUUAAGCAAGUUGUUUUCCGCUAAUGUCAGGUCUCACUACUUUUUGCUGCUAUUUUUCUUCGCUCAUGGUUUCUUCAUAAGGCGUUUUUAUGAUGGUUUUUCGAAAUUGGUUUUUGAGACGACGGUUGCUCAAGGUUAUUGUUUUUGUUUUCUUCUGGUUGUUUUCUAUUUUCUUUUUUUUAGCUUUCUGUUUCUCCCUUAGUUUGGCUUUUUGCUUCAUACUCUUCCCUGUCUUUCCGAGCCGUUUAUGUCCAACGCGGGAUUUGGUUUUUCUUUAUCGAUGGGAAGAAAUGGUGCUAUAGUAGGUUGGGAGAUAAUAUUUAUGGUAUGGGGUGCUAGUGCGGAUGGGGCGCUCUUAUUGUUGAUUUCUUCGCUCGUCUUCUUUUUCUGGUGGCGCUGCAAGAGGAAGUUUUUCGACUUUGUUAUGAUUUUUGGUUUGCAAGGAAAGGUGUCUUACGAUUCUUUUUUUGAUGUAAUAGGAUAAGCUUGCUUAUCCCCCAAGUAUCGGCCAAAGUUGUUGAUUUUCCUUUUGAAGUGUCCUCGGUUUGAGGGGGUGUAGGGUGGGGUUGGUCUACAAUAAGAGUGUUCCAUUGUUAACGUGCUGGCGUCUUUUACUAUAUUUUUUUUCCCAGUUUAUUUUGUGCUUAUUUUCUCAUUGAGGAGAAGGAGCUCUUCUCGCAGGAUAUAAAUGGAGGUUUGCUAAAGGGGAGGAGAUGUGUUUGUGAGAAUACUGCUGAGAGAGUUCUGGAAGAGAAAAAAAGGAGGCAAUGGAAGGCGUUUGCUGGGAAAAGAGAAGAGCCAUGACUGCAUCUGUUGUUUCAAGGCCAGUUUUAUUAACCGCCUAUGUCAUAGAGGCGUUUUUUUUGGAGGGAUUUGAAGAAUGCCGGCGGCAUCAAGAAACGGACUUGAUGGUUGACGCCUGUUUUUAAAGUUAGAGACGUCGCGACCCUCGCACUUGUGGAGUCGUUCUUGACUUUUACUUUGGUCGCUUGAUGUUUCUCUCGUCUUCCCGUUCGCUCUU
Lig16 RNA GUAUGUAUUUAUUUUAGAACUAGUUACUAACAUUUUUUUUUUAAAAAAUAAUUAUAUAG
Lig218 RNA GAGAGAUUCCGUACACCAUCAGGGUACGAGCUAGCCCAUGGCGUACACCAUCAGGGUACGACUAGUAGAUCUCGUACACCAUCAGGGUACGGAAUUCUCUAGAGUGUCGACGAACUAAGUGAUCUAGAAAGGUAUGUCUAAAGUUAUGGCCACGUUUCAAAUGCGUGCUUUUUUUUUAAAACUUAUGCUCUUAUUUACUAACAAAAUCAACAUGCUAUUGAACUAGAGAUCCACCUACUUCAUGUU
Lig41 RNA GUUCGCGAAGUAACCCUUCGUGGACAUUUGGUCAAUUUGAAACAAUACAGAGAUGAUCAGCAGUUCCCCUGCAUAAGGAUGAACCGUUUUACAAAGAGAUUUAUUUCGUUUU
Lig76 RNA GUAUGUAUUUAUUUU
Lig90 RNA AUAAAUUUUUAAGNGUAUGUAUUUAUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUAAGAACUAGAUACUAACACUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUUAAAUAGUAAAU
Lig96 RNA GUAUGUAUUUAUUUUNNNNNNNNNNUAGAUACUAACAC